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Histochemical characterization of duodenum at early, mid and late prenatal period in goats

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Title Histochemical characterization of duodenum at early, mid and late prenatal period in goats
 
Creator Verma, Abhinov
Farooqui, Muneer Mohammad
Mohammad, Muneer
Pathak, Archana
Singh, Shriprakash
Gupta, Varsha
 
Subject Bound lipids
Capra aegagrus hircus (L.)
Feulgen reaction
Livestock
Mucopolysaccharides
 
Description 939-945
Goats [Capra aegagrus hircus (L.)] play a significant role in
providing supplementary income and livelihood to humans. The
intestine plays a major role in foetus development and growth,
and duodenum, as the part of small intestine, is responsible for
breakdown of food. As there is not much studies available in
literature on this aspect, here, we investigated the developing
duodenum of 30 goat embryos/foeti irrespective of breed and sex.
The tissues were fixed in 10% neutral buffered formalin and in
cold acetone. Different histochemical techniques were applied for
the detection and localization of neutral mucopolysaccharides,
acid mucopolysaccharides, bound lipids, alkaline phosphatase
enzymes, acid phosphatase enzymes, and DNA. The intensity of
reactions increased as the age of foeti advanced. The goblet cells
of the intestinal gland showed moderate reaction for Periodic acid
schiff (PAS) and Acid mucopolysaccharides (AMPs) in the mid
prenatal period (Gr. II) and intense to highly intense reaction in
the late prenatal period (Gr. III). The cytoplasm of the epithelial
cells exhibited negative to mild reaction for bound lipids in Gr. I,
mild to moderate in Gr. II and mild to intense reaction in Gr. III.
The luminal border of epithelial cells exhibited mild reaction in
Gr. I & II, and moderate to intense reaction in Gr. III. The luminal
border showed weak acid phosphatase reaction in Gr. I & II, and
mild to moderate reaction in Gr. III. The nuclei of the epithelial
cells showed mild to moderate Feulgen reaction in Gr. I & II, and
intensely positive reaction in Gr. III.
 
Date 2022-12-02T06:07:00Z
2022-12-02T06:07:00Z
2022-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/60987
https://doi.org/10.56042/ijeb.v60i12.56161
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.60(12) [Dec 2022]