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Ectopic overexpression of cytosolic ascorbate peroxidase gene (Apx1) improves salinity stress tolerance in Brassica juncea by strengthening antioxidative defense mechanism

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Title Ectopic overexpression of cytosolic ascorbate peroxidase gene (Apx1) improves salinity stress tolerance in Brassica juncea by strengthening antioxidative defense mechanism
 
Creator Saxena, Saurabh C.
Salvi, Prafull
Kamble, Nitin Uttam
Joshi, Pankaj K.
Majee, Manoj
Arora, Sandeep
 
Subject Salinity stress
Brassica juncea
Reactive oxygen species
Transgenic plants
Antioxidant system
 
Description Accepted date: 05 March 2020
Salinity stress is considered to be a key constrain that reduces the crop productivity by impairing plant growth and development. During salt stress condition, an underlying mechanism for reduction in crop yield is increase in ROS level that can potentially harm cellular macromolecules, leading to disruption of essential physiological and biochemical processes. Plants possess a complex antioxidative defense machinery for scavenging these ROS. Ascorbate peroxidase (APX, E.C. 1.11.1.11), is a crucial antioxidant enzyme involved in Ascorbate–Glutathione pathway that primarily detoxifies the negative impact of H2O2 in cell. The efficient scavenging of H2O2 is a prerequisite for enhanced tolerance to salinity stress. Here, we have inspected whether over-expression of APX could provide protection against salinity stress. Cytosolic ascorbate peroxidase (Apx1) gene, isolated from Arabidopsis thaliana, was chosen as the candidate gene for strengthening the antioxidative defense system of Brassica juncea. Physiological parameters were employed to analyze the growth status of transgenic plants. Leaf disc assay was done to evaluate the salinity stress tolerance potential of transgenic plants, using several physiological and biochemical parameters. Under salinity stress, the transgenic plants performed well as compared to their non-transgenic counterparts; as revealed through greater proline accumulation, increased chlorophyll stability index, lower chlorophyll a/b ratio, and higher antioxidative enzyme activities. Further, the lower H2O2 levels were well correlated with lesser membrane damage as measured through MDA content. Collectively, our results clearly depicted that ectopic overexpression of AtApx1 gene was able to confer salinity stress tolerance by strengthening the antioxidative defense system in B. juncea.
SCS thank the Council of Scientific and Industrial Research (CSIR), Government of India, for research fellowship. Technical assistance from Mr. Lalit is acknowledged.
 
Date 2020-03-31T14:01:40Z
2020-03-31T14:01:40Z
2020
 
Type Article
 
Identifier Acta Physiologiae Plantarum, 42: 45
1861-1664
https://doi.org/10.1007/s11738-020-3032-5
https://link.springer.com/article/10.1007/s11738-020-3032-5
http://223.31.159.10:8080/jspui/handle/123456789/1050
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature