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Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I
 
Creator Kakkar, Saloni
Chauhan, Sakshi
Bala, Rajni
Bharti, Bala
Kumar, Virendra
Rohit, Manojkumar
Bhalla, Vijayender
 
Subject QR Microbiology
 
Description Bioreceptor functionalized metallic nano-colloids have been identified as effective nanobioprobes to realize the detection of an analyte based on a common phenomenon of salt-induced aggregation. In marked contrast to this, we describe a nano-sandwich assay integrating the novel match-pair of aptamer and peptide functionalized gold nanoparticles. The site-directed biomolecular interaction of high affinity aptamer and peptide bioreceptors directed towards distinct sites of cardiac biomarker troponin I; this was found to form a nano-sandwich assay in a peculiar manner. The gold nanoconjugates interact with specific and distant regions of troponin I to result in collision of probes upon target identification. In the presence of TnI, both nanobioprobes bind at their respective sites forming a nano-sandwich pair providing a visual color change from red to blue. Thus, the presence of target TnI itself causes instant agglomeration in just a single-step without addition of any external aggregator. The assay imparts 100% specificity and 90% sensitivity in a dynamic concentration range of 0.1-500 ng/mL troponin I with detection limit as low as 0.084 ng/mL. The applicability of the assay has been validated in clinical samples of acute myocardial infarction patients thus establishing a promising point-of-care detection of TnI.
 
Publisher Springer
 
Date 2022-10
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s00604-022-05461-9
http://crdd.osdd.net/open/3029/
 
Identifier Kakkar, Saloni and Chauhan, Sakshi and Bala, Rajni and Bharti, Bala and Kumar, Virendra and Rohit, Manojkumar and Bhalla, Vijayender (2022) Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I. MICROCHIMICA ACTA, 189 (10).