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Isolation, purification, and characterization of a novel exopolysaccharide isolated from marine bacteria Brevibacillus borstelensis M42

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Isolation, purification, and characterization of a novel exopolysaccharide isolated from marine bacteria Brevibacillus borstelensis M42
 
Creator Srivastava, Nandita
Kumari, Sumeeta
Kurmi, Shubham
Pinnaka, Anil Kumar
Choudhury, Anirban Roy
 
Subject QR Microbiology
 
Description Marine microbes produce polysaccharides with unique physicochemical and functional properties that help them survive in harsh marine environments. However, only a handful of marine exopolysaccharides (EPSs) have been reported to date. The present study explored the seashore of Visakhapatnam, India, to report a novel exopolysaccharide designated as Br42 produced by Brevibacillus borstelensis M42. The isolate was identified through morphological, biochemical, phylogenetic, and genome sequencing analysis. The studies on fermentation kinetics revealed that EPS Br42 was a primary metabolite with a maximum production of 1.88 ± 0.02 g/L after 60 h when production broth was fortified with 2% glucose. Additionally, EPS Br42 was found to be a heteropolysaccharide consisting of glucose and galacturonic acid with a molecular weight of about 286 kDa. Interestingly, this molecule possesses industrially relevant functional properties such as water-holding (510 ± 0.35%), oil-holding (374 ± 0.12% for coconut oil and 384 ± 0.35% for olive oil), and swelling capacities (146.6 ± 5.75%). EPS Br42 could form an emulsion that was stable at a wide pH range for about 72 h and, in fact, performed better as compared to Span 20, a commercially used synthetic emulsifier. Moreover, this EPS was also found to be heat stable and exhibited non-Newtonian pseudoplastic behavior. These physicochemical and functional properties of polysaccharides suggest that the EPS Br42 has potential for multifarious industrial applications as an emulsifier, stabilizer, viscosifier, and binding agent.
 
Publisher Springer
 
Date 2022-06-17
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s00203-022-02993-9
http://crdd.osdd.net/open/3053/
 
Identifier Srivastava, Nandita and Kumari, Sumeeta and Kurmi, Shubham and Pinnaka, Anil Kumar and Choudhury, Anirban Roy (2022) Isolation, purification, and characterization of a novel exopolysaccharide isolated from marine bacteria Brevibacillus borstelensis M42. ARCHIVES OF MICROBIOLOGY, 204 (7).