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Nisin Production in a Two Liters Bioreactor Using Lactococcus lactis NCIM 2114

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Title Nisin Production in a Two Liters Bioreactor Using Lactococcus lactis NCIM 2114
Not Available
 
Creator Sunita Singh
Sukanta Dash
K. Rinu
S. Saha
S. Gupta
S. Mandjiny
D. Upadhyay
L Holmes
 
Subject Nisin
Food preservative
RP-UFLC
Fermentation technology
Bioreactor, Bioactivity
Cell-free extract
Box Behnken
 
Description Not Available
The facultative anaerobe, Lactococcus lactis was grown in batch fermentation (1.5 L working volume) at 30°C, using 5% inoculums to initiate growth for the production of the antimicrobial food additive nisin. L. lactis strain NCIM 2114 produced a maximal nisin concentration of 50,400 IU/mL and 8,830 IU/mg biomass in MRS growth medium using a 2 L bioreactor with optimal conditions of 25 rpm and 0.2 vvm determined by a Box Behnken design statistical package. Nisin was obtained from cell-free extracts and quantified using ultra-fast liquid chromatography (UFLC). Maximum bioactive nisin concentration was found at 4 h and harvested. Increasing aeration, agitation or time until harvest had negative impact on bioactive product yield. In a repeated trial under the same conditions, cell-free extracts of nisin were measured by well diffusion assay at 240 IU/mL (antimicrobial activity). Bioactive nisin was confirmed. The optimal specific growth rate for L. lactis was measured to be 0.25 h-1. This study shows that active biomass production can be increased using conditions determined by standard statistical methods.
Not Available
 
Date 2019-05-02T05:34:21Z
2019-05-02T05:34:21Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19116
 
Language English
 
Relation Not Available;
 
Publisher Not Available