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Identification, characterization, validation and cross-species amplification of genic-SSRs in Indian Mustard (Brassica juncea)

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Title Identification, characterization, validation and cross-species amplification of genic-SSRs in Indian Mustard (Brassica juncea)
Not Available
 
Creator Binay K. Singh
Dwijesh C. Mishra
Sushma Yadav
Supriya Ambawat
Era Vaidya
Kishor U. Tribhuvan
Arun Kumar
Sujith Kumar
Sanjeev Kumar
K.K. Chaturvedi
Reema Rani
Prashant Yadav
Anil Rai
P.K. Rai
V.V. Singh
Dhiraj Singh
 
Subject Brassica juncea
Transcriptome
Simple sequence repeats
Cross-species amplification
 
Description Not Available
Brassica juncea is an economically important oilseed crop worldwide. It has limited genomic resources at present. We generated 47,962,057 expressed sequence reads which were assembled into 45,280 unigenes. A total of 4108 SSR loci (≥10 bp) were identified in these unigenes. Trinucleotide was the most frequent repeat unit (59.91 %) followed by di- (38.66 %), tetra - (0.71 %), hexa - (0.49 %) and pentanucleotide repeats (0.24 %). Primers were designed for 2863 SSR loci among which 460 were selected for primer synthesis. A total of 339 loci amplified successfully of which 134 (39.5 %) exhibited polymorphism among six B. juncea genotypes with PIC values ranging from 0.18 to 0.81. Further, 25 polymorphic SSRs were used for analysis of genetic variability in 25 genotypes of Brassicas and their wild relatives. Two to five alleles with PIC values 0.22–0.66 were detected at these loci. The dendrogram grouped the genotypes according to their known pedigree/systematic position.
Not Available
 
Date 2018-02-22T04:18:44Z
2018-02-22T04:18:44Z
2016-03-23
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/5843
 
Language English
 
Relation Not Available;
 
Publisher Society for Plant Biochemistry and Biotechnology, Springer