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Diversity of Bacillus thuringiensis Isolates Native to Uttarakhand Himalayas, India and Their Bioefficacy Against Selected Insect Pests

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Title Diversity of Bacillus thuringiensis Isolates Native to Uttarakhand Himalayas, India and Their Bioefficacy Against Selected Insect Pests
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Creator A. R. N. S. Subbanna • M. S. Khan • J. Stanley • B. Kalyana Babu
 
Subject Bacillus thuringiensis Uttarakhand Himalayas Gene profiles Diversity Bioassay
 
Description Not Available
The huge family status and diversified agricultural
applications of Bacillus thuringiesnsis (Bt) Berliner
made the bacterium, a highly specialized bio-insecticide
with worldwide commercial usage. Novel strains and cry
genes always expand potential usage of the bacterium. In
the present investigation, the authors isolated 45 candidate
strains of Bt from a unique ecological niche of Indian
subcontinent, i.e., Uttarakhand state belongs to North
Western Himalayas of India. All the isolates were characterized
for light microscopy, PCR, SDS-PAGE and
bioassays against major insect pests. The diversity in collection
is manifested by existence of two major crystal
shapes (Bipyramidal and spherical) and 21 different cry
gene profiles with predominance of cry1 (cry1Aa, cry1Ab,
cry1Ac, cry1B, cry1C, cry1D, cry1I) family genes followed
by cry2 (cry2A, cry2B) and cry26. Interestingly, coleopteran
specific genes were governed by cry8 not the cry3.
The sequencing of major cry genes showed preferential
divergence of cry2A genes and led to partial identification
of novel cry1D gene from UKBt13. Majority of the isolates
showed existence of 68 and 130 kDa crystal toxins. The
bioefficacy studies presented here led us to identify putative
novel strains with potential applications in pest management
programs and associated toxicological diversity of
Bt in Uttarakhand Himalayas.
Not Available
 
Date 2018-11-17T10:43:35Z
2018-11-17T10:43:35Z
2017-07-02
 
Type Article
 
Identifier Not Available
0369-8211
http://krishi.icar.gov.in/jspui/handle/123456789/11769
 
Language English
 
Relation Not Available;
 
Publisher Not Available