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Rapid screening technique for Alternaria blight resistance in Indian mustard (Brassica juncea L.) using cotyledonary leaf method

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Title Rapid screening technique for Alternaria blight resistance in Indian mustard (Brassica juncea L.) using cotyledonary leaf method
Not Available
 
Creator Meena P.D., S.J. Jambhulkar, Riteka Gupta, H.S. Meena and Dhiraj Singh
 
Subject Alternaria blight, Brassica juncea, cotyledonary leaf, resistance, screening technique, rating scale
 
Description Not Available
Alternaria blight (AB) caused by Alternaria brassicae
(Berk.) Sacc. is a devastating disease of oilseed Brassicas
all over the world, and responsible for significant seed
yield losses up to 47%. No reliable, resistant germplasm is
available to develop AB resistant cultivars. Various screening
techniques have been reported so far, but cotyledonary
leaf method is not yet reported. Three methods were
tested using one susceptible cultivar (Varuna): inoculation
of seed, inoculation of cotyledons, and inoculation of both
seed and cotyledons. Fungal conidia were inoculated directly
onto the seedlings with 1.5 × 105, 2.5 × 105, 4 × 105
and 5 × 105 conidia ml−1 concentrations for standardization.
Percentage AB severity increased with the increase in conidial
concentration, therefore the highest concentration
was used for final screening. Among the three screening
methods, inoculation of both seed, and cotyledon method
was found highly effective where mean AB severity
on cotyledon was 84.6% in comparison to 49.3% in the
inoculation of seed and 62.5% in the inoculation of cotyledon
methods. The technique was validated by screening
susceptible and putative tolerant genotypes. The severity
of AB was 54% of susceptible cultivar and 16.4%-21.2% of
tolerant genotypes. The conidia number per microscopic
field was 21.5 in putative tolerant and 43.5 in susceptible
genotypes. Thus, in vitro screening of AB using inoculation
of both seed and cotyledon method was found most
effective and could be used for rapid screening in early
stages of plant growth. A new 0-7 rating scale was also
devised to observe the AB pathogen interaction phenotype
at the cotyledonary stage of oilseed Brassica.
Not Available
 
Date 2018-10-30T06:54:45Z
2018-10-30T06:54:45Z
2016-11-15
 
Type Journal
 
Identifier Meena P.D., S.J. Jambhulkar, Riteka Gupta, H.S. Meena and Dhiraj Singh. 2016. Rapid screening technique for Alternaria blight resistance in Indian mustard (Brassica juncea L.) using cotyledonary leaf method. Journal of Plant Pathology 98 (3): 463-469. DOI: 10.4454/JPP.V98I3.033.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/8616
 
Language English
 
Relation Not Available;
 
Publisher Edizioni ETS Pisa