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Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.)

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Title Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.)
Not Available
 
Creator Binay K. Singh, Divakar Nandan, Supriya Ambawat, Bhagirath Ram, Arun Kumar, Tawar Singh, H. S. Meena, Vinod Kumar, V. V. Singh, P. K. Rai, and Dhiraj Singh
 
Subject White rust, marker-assisted selection, marker validation
 
Description Not Available
Singh, B. K., Nandan, D., Supriya, A., Ram, B., Kumar, A., Singh, T., Meena, H. S., Kumar, V., Singh, V. V., Rai, P. K.
and Singh, D. 2015. Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian
Mustard (Brassica juncea L.). Can. J. Plant Sci. 95: 939 945. Successful application of molecular markers in markerassisted
pyramiding relies on effective determination of the target phenotype. In this respect, evaluation of the efficiency of
markers for marker-assisted selection through cross-validation in different genetic backgrounds and in different
populations is a crucial step. In the present study, the previously identified Arabidopsis-derived intron polymorphic (IP)
markers At5g41560 and At2g36360, which were highly linked with AcB1-A4.1 and AcB1-A5.1, respectively, were validated
in a set of 25 genotypes of Indian Mustard and in three different F2 populations. The relationships between the variation of
PCR products of the two markers with the percent disease index (PDI) of the tested genotypes, and the co-segregation
analysis of the markers with disease phenotype in F2 populations clearly indicated that At5g41560 and At2g36360 are
genotype-nonspecific markers and are closely linked to white rust resistance loci AcB1-A4.1 and AcB1-A5.1, respectively.
It also became evident from the present study that AcB1-A4.1 and an another white rust resistance locus Ac(2)t are likely
the same gene locus.
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Date 2018-11-19T09:28:31Z
2018-11-19T09:28:31Z
2015-05-21
 
Type Article
 
Identifier 2
http://krishi.icar.gov.in/jspui/handle/123456789/12063
 
Language English
 
Relation Not Available;
 
Publisher Canadian Journal of Plant Science