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RT-PCR detection of Potato leaf roll virus (PLRV) in aphids from Northern and North-Eastern India

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Title RT-PCR detection of Potato leaf roll virus (PLRV) in aphids from Northern and North-Eastern India
Not Available
 
Creator Baswaraj Raigond
Vallepu Venkateswarlu
J Sridhar
A Jeevalatha
Sanjeev Sharma
B P Singh
 
Subject RT-PCR, PLRV, coat protein, mt COI, aphid, Myzus persicae
 
Description Not Available
A reverse transcription polymerase chain reaction (RT-PCR) assay was developed to detect Potato leaf roll virus
(PLRV) in aphids collected from potato fields. Total RNA was isolated from aphids and cDNA was synthesized
with the help of random primers. In-house optimized PCR protocol for detection of PLRV targeting coat protein
(CP) gene was adopted. Primers targeting cytochrome oxidase I (COI) were designed and PCR conditions were
optimized so as to match with detection protocol of PLRV. However, before adopting the above optimized technique
for routine detection of PLRV in aphids, the respective PCR amplified genes of PLRV (CP) and aphid (COI) were
gel eluted and sequenced directly. The BLAST results revealed that both the queries showed a homology of 99 per
cent each with PLRV and Myzus persicae. By adopting the developed RT-PCR technique, PLRV was successfully
detected in the aphid samples collected from Shillong (Meghalaya) and Jalandhar (Punjab). Hence, this optimized
technique was used to screen the aphids collected from different geographical locations for their viruliferousness
with respect to PLRV. However, the virus was not present in aphids from other locations
Not Available
 
Date 2018-11-23T05:46:58Z
2018-11-23T05:46:58Z
2014-11-15
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/12911
 
Language English
 
Relation Not Available;
 
Publisher Not Available