Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep.
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep.
Not Available |
|
Creator |
Mohanty NN
Hemadri D Munivenkatarayappa A Shetty N Subramanyam V Biswas SK Chanda MM Shivachandra SB |
|
Subject |
Bluetongue
Diagnosis Indirect ELISA Non-structural protein Sero-surveillance |
|
Description |
Not Available
Bluetongue is an insect borne (Culicoides) viral disease of small ruminants. The virus blankets the globe with a wide serotypic variation, numbered from 1 to 28. In India 21 different serotypes have been reported to be circulating across the various agro-climatic zones of the country. Non-structural proteins (NSPs) of bluetongue virus have always remained ideal target for differentiation of infected from vaccinated animals. The current study is an extrapolation of our previous work where a novel fusion construct comprising of bluetongue viral segment NS1 and NS3 was successfully cloned, expressed, purified with an efficient strategy for its suitable implementation as a diagnostic antigen. In this study, the applicability of the fusion construct has been further evaluated and optimised for field applicability. The fusion construct used in an ELISA platform projected a relative diagnostic sensitivity and specificity of 98.1% and 95.5% respectively against a pre-established test panel. The rNS1-NS3 ELISA showed substantially good agreement with the commercial BTV antibody detection kit. Finally, the study brings together the diagnostic capability of two NSPs, which can be a handy tool for sero-surveillance of bluetongue. Not Available |
|
Date |
2022-04-18T09:25:06Z
2022-04-18T09:25:06Z 2021-01-04 |
|
Type |
Research Paper
|
|
Identifier |
Mohanty NN, Hemadri D, Munivenkatarayappa A, Shetty N, Subramanyam V, Biswas SK, Chanda MM, Shivachandra SB. Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep. J Immunol Methods. 2021 Mar;490:112959. doi: 10.1016/j.jim.2021.112959. Epub 2021 Jan 4. PMID: 33412173.
0022-1759 http://krishi.icar.gov.in/jspui/handle/123456789/71573 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Elsevier Publishing
|
|