Record Details

Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep.
Not Available
 
Creator Mohanty NN
Hemadri D
Munivenkatarayappa A
Shetty N
Subramanyam V
Biswas SK
Chanda MM
Shivachandra SB
 
Subject Bluetongue
Diagnosis
Indirect ELISA
Non-structural protein
Sero-surveillance
 
Description Not Available
Bluetongue is an insect borne (Culicoides) viral disease of small ruminants. The virus blankets the globe with a wide serotypic variation, numbered from 1 to 28. In India 21 different serotypes have been reported to be circulating across the various agro-climatic zones of the country. Non-structural proteins (NSPs) of bluetongue virus have always remained ideal target for differentiation of infected from vaccinated animals. The current study is an extrapolation of our previous work where a novel fusion construct comprising of bluetongue viral segment NS1 and NS3 was successfully cloned, expressed, purified with an efficient strategy for its suitable implementation as a diagnostic antigen. In this study, the applicability of the fusion construct has been further evaluated and optimised for field applicability. The fusion construct used in an ELISA platform projected a relative diagnostic sensitivity and specificity of 98.1% and 95.5% respectively against a pre-established test panel. The rNS1-NS3 ELISA showed substantially good agreement with the commercial BTV antibody detection kit. Finally, the study brings together the diagnostic capability of two NSPs, which can be a handy tool for sero-surveillance of bluetongue.
Not Available
 
Date 2022-04-18T09:25:06Z
2022-04-18T09:25:06Z
2021-01-04
 
Type Research Paper
 
Identifier Mohanty NN, Hemadri D, Munivenkatarayappa A, Shetty N, Subramanyam V, Biswas SK, Chanda MM, Shivachandra SB. Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep. J Immunol Methods. 2021 Mar;490:112959. doi: 10.1016/j.jim.2021.112959. Epub 2021 Jan 4. PMID: 33412173.
0022-1759
http://krishi.icar.gov.in/jspui/handle/123456789/71573
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Publishing