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Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis

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Title Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis
Not Available
 
Creator Nagalingam M
Basheer TJ
Balamurugan V
Shome R
Kumari SS
Reddy GBM
Shome BR
Rahman H
Roy P
Kingston JJ
Gandham RK
 
Subject Brucella abortus
Brucella lumazine synthase
Brucella protein26
Cu-Zn superoxide dismutase
Yersinia enterocolitica O:9
cattle brucellosis
 
Description Not Available
Background and aim: The present serodiagnosis of brucellosis in livestock is based on the whole cell or smooth lipopolysaccharide of the Brucella organism in which specificity is hampered by the cross-reactivity, especially with the antibodies against Yersinia enterocolitica O:9 organism. The problem can be addressed by screening for better immunodominant antigens. Hence, the present study was undertaken to screen protein antigens of Brucella abortus for their diagnostic potential in cattle brucellosis. Materials and methods: Protein antigens of B. abortus (n=10) non-reactive to antibodies against Y. enterocolitica O:9 were selected, expressed in Escherichia coli, assessed the reactivity of expressed recombinant proteins by Western blot, standardized indirect-enzyme-linked immunosorbent assay (ELISA) for detecting Brucella antibodies in cattle serum, and comparative evaluation was done. Results: All the selected protein antigens were expressed and in the Western blot with Brucella antibodies positive cattle serum, six recombinant (Brucella protein 26 [BP26], Cu-Zn Superoxide dismutase [SodC], B. abortus I-1885, Serine protease, Bacterioferritin, and Brucella Lumazine Synthase [BLS]) proteins showed reaction whereas none of the proteins showed reactivity with Brucella negative cattle serum. ELISA has been done using known Brucella positive and negative cattle sera samples (n=113 each) in which the performance of recombinant proteins in diagnosing brucellosis was in the order of BP26 > BLS > SodC followed by rest of the proteins. BP26 based ELISA was found to be better with area under the curve as 0.953, and diagnostic sensitivity, diagnostic specificity, and Youden's index of 90.27%, 95.58%, and 0.8584, respectively, with the excellent agreement (k=0.85). Conclusion: BP26 could be a potential diagnostic antigen among the immunodominant proteins of B. abortus in ruling out Y. enterocolitica O:9 infection while diagnosing brucellosis in cattle herds.
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Date 2022-04-18T09:26:43Z
2022-04-18T09:26:43Z
2021-03-30
 
Type Research Paper
 
Identifier Nagalingam M, Basheer TJ, Balamurugan V, Shome R, Kumari SS, Reddy GBM, Shome BR, Rahman H, Roy P, Kingston JJ, Gandham RK. Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis. Vet World. 2021 Mar;14(3):803-812. doi: 10.14202/vetworld.2021.803-812. Epub 2021 Mar 30. PMID: 33935431; PMCID: PMC8076471.
2231-0916
http://krishi.icar.gov.in/jspui/handle/123456789/71576
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World