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Molecular identification and phylogenetic analyses of multiple nucleopolyhedrovirus isolated from Lymantria obfuscate (Lepidoptera: Lymantriidae) in India

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Title Molecular identification and phylogenetic analyses of multiple nucleopolyhedrovirus isolated from Lymantria obfuscate (Lepidoptera: Lymantriidae) in India
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Creator Gani M, Gupta R K, Zargar SM, Kour G, Monobrullah Md, Kandasamy T, Mohanasundaram A
 
Subject Baculoviruses · Genetic variation · Phylogeny · Pathogenicity
 
Description Not Available
The Lymantria obfuscata Walker (Lyob) multiple (M) nucleopolyhedrovirus (NPV) (LyobMNPV) has been isolated and successfully applied for the management of the Indian gypsy moth, L. obfuscata in Jammu and Kashmir (J&K), India. The present work aimed to investigate the variability of LyobMNPV isolates from six localities of J&K through molecular [amplification of the polyhedrin (polh), late expression factor - 8 (lef - 8) and late expression factor - 9 (lef - 9) genes] and biological (bioassays) characterization. To identify the position of LyobMNPV in the phylogenetic tree of baculoviruses, partial sequences of the polh, lef - 8 and lef - 9 genes were determined by using the DNA sequences within their coding regions by optimizing the polymerase chain reaction with degenerate primers. The sequence alignment revealed that LyobMNPV isolates exhibited seven, five and eleven single nucleotide polymorphic sites in the case of polh, lef - 8 and lef - 9, respectively. The phylogenetic analyses supported placing LyobMNPV with the Lymantria dispar L. MNPV (LdMNPV) isolates from different countries, and showed that it was more closely related to LdMNPV than to Lymantria xylina Swinhoe NPV and Lymantria monacha L. NPV. The contaminated diet plug bioassays using 2nd instar larvae indicated that the median lethal dose (LD50) and median survival time (ST50) of different isolates of LyobMNPV against L. obfuscata were lower than those of LdMNPV against L. dispar. LyobMNPV was more closely related to LdMNPV but its LD50 and ST50 were lower than those of LdMNPV. The study provides novel information on the position of LyobMNPV in the phylogenetic tree of baculoviruses and about biological and genetic variation of Lymantria species’ NPV isolates from different parts of the world.
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Date 2020-09-22T05:05:40Z
2020-09-22T05:05:40Z
2017-04-20
 
Type Research Paper
 
Identifier Gani, M., Gupta, R. K., Zargar, S. M., Kour, G., Monobrullah, M., Kandasamy, T., & Mohanasundaram, A. (2017). Molecular identification and phylogenetic analyses of multiple nucleopolyhedrovirus isolated from Lymantria obfuscata (Lepidoptera: Lymantriidae) in India. Applied Entomology and Zoology, 52(3), 389-399.
0003-6862
http://krishi.icar.gov.in/jspui/handle/123456789/41473
 
Language English
 
Relation Not Available;
 
Publisher Springer