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Isolation and characterization of an oxygen mutant of Azorhizobium caulinodans.

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Title Isolation and characterization of an oxygen mutant of Azorhizobium caulinodans.
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Creator Das Avijit and Lodha ML
 
Subject oxygen sensitive, Azorhizobium caulinodans, azide tolerance, respiration rate, oxygen regulation, nitrogen fixation
 
Description Doctoral thesis work.
An oxygen sensitive mutant of Azorhizobium caulinodans strain IRBG 46 was isolated by NTG mutagenesis. It was defective in N2 fixation under 3% O2 level, while under 1% O2 it was almost as active as the parent strain IRBG 46. The mutant was also found to be a slow grower with reduced respiratory activity, low azide tolerance and no catalase activity. However, it did not differ from its parent strain with respect to nitrate respiration. Under symbiotic condition the mutant formed smaller, light green nodules as compared to bigger, dark green nodules formed by the wild type strain. The mutant was also defective in N2 fixation under symbiotic condition. Complementation analysis showed that the mutation might be in either tixl or tixJ gene which are involved in O2 regulation of nit/fix gene expression. A possible role of all these factors in conferring a highly O2 tolerant nitrogen fixing system in the organism, has been discussed.Not Available.
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Date 2021-04-30T05:57:10Z
2021-04-30T05:57:10Z
1997-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/46877
 
Language English
 
Relation Not Available;
 
Publisher Society for Plant Biochemistry and Biotechnology