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Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials

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Title Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
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Creator Rajiv Kumar, D.B. Shakyawar, P.K. Pareek, A.S.M. Raja, L.L.L. Prince, Satish Kumar and S.M.K. Naqvi
 
Subject Pashmina. Cottage-based industry. Fiber identification, PCR, Textile materials
 
Description Not Available
Pashmina fiber is one of major specialty animal fiber in India. The quality of Pashmina obtained from Changthangi and Chegu goats in India is very good. Due to restricted availability and high prices, adulteration of natural prized fibers is becoming a common practice by the manufacturers. Sheep wool is a cheap substi-tute, which is usually used for adulteration and false declaration of Pashmina-based products. Presently, there is lack of cost-effective and readily available methodology to identify the adulteration of Pashmina products from other similar looking substitutes like sheep wool. Polymerase chain reaction (PCR)-based detection method can be used to identify origin of animal fiber. Extraction of quality DNA from dyed and processed an-imal fiber and textile materials is a limiting factor in the development of such detection methods. In the pre-sent study, quality DNA was extracted from textile materials, and PCR-based technique using mitochondrial gene (12S rRNA) specific primers was developed for detection of the Pashmina in textile blends. This tech-nique has been used for detection of the adulteration of the Pashmina products with sheep wool. The tech-nique can detect adulteration level up to 10 % of sheep/goat fibers in textile blends.
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Date 2021-06-08T06:14:54Z
2021-06-08T06:14:54Z
2015-02-21
 
Type Research Paper
 
Identifier Not Available
0273-2289
http://krishi.icar.gov.in/jspui/handle/123456789/47141
 
Language English
 
Relation Not Available;
 
Publisher Springer