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Modified method of DNA isolation and optimization of PCR protocol for RAPD analysis of endangered medicinal plant Nardostachys jatamansi D C
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View Archive Info| Field | Value | |
| Title |
Modified method of DNA isolation and optimization of PCR protocol for RAPD analysis of endangered medicinal plant Nardostachys jatamansi D C
Not Available |
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| Creator |
Uma M. Singh , Dinesh Yadav , Manoj Kumar Tripathi , Waseem Seikh, Manoj K. Yadav
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| Subject |
Dry rhizome, DNA extraction, RAPD, Phenols, Polysaccharides
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| Description |
Not Available
An important but limiting step in any molecular biological work is the reliable method of DNA isolation and suitable protocol for PCR analysis. Due to abundance of phenols, polysaccharides, terpenoids and other secondary metabolites in the rhizome of Jatamansi, it is a major problem to get high quality DNA. To isolate quality DNAfor PCR analysis modified protocol developed in our lab to overcome such serious problems. We tried to optimize the concentration of PVP, â-mercaptoethanol and NaCl. Upon gel documentation of isolated DNA by modified method evinced single discrete band of genomic DNA and yielded significantly superior, 30-50 µg/g DNA from dry rhizome. The composition of PCR master mix was also modified for RAPD analysis which includes changing the concentration of MgCl and observed a good amplification. Reproducible amplifiable products were 2 observed in all accessions. Not Available |
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| Date |
2021-08-19T05:30:06Z
2021-08-19T05:30:06Z 2004-12-01 |
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| Type |
Research Paper
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| Identifier |
Not Available
2321 – 3663 http://krishi.icar.gov.in/jspui/handle/123456789/58760 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Scientific Planet Society
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