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Overexpression of GmCaM4 in Soybean Enhances Resistance to Pathogens and Tolerance to Salt Stress

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Title Overexpression of GmCaM4 in Soybean Enhances Resistance to Pathogens and Tolerance to Salt Stress
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Creator SURYADEVARA S. RAO, MOHAMED H. EL-HABBAK, WENDY M. HAVENS, AJAY SINGH, DANMAN ZHENG, LAURA VAUGHN, JAMES S. HAUDENSHIELD, GLEN L. HARTMAN3, SCHUYLER S. KORBAN AND SAID A. GHABRIAL
 
Subject GmCaM4, soybean, pathogens,salt stress
 
Description Not Available
Plant diseases inflict heavy losses on soybean yield, necessitating
an understanding of the molecular mechanisms underlying biotic/
abiotic stress responses. Ca2+ is an important universal messenger,
and protein sensors, prominently calmodulins (CaMs), recognize
cellular changes in Ca2+ in response to diverse signals. Because the
development of stable transgenic soybeans is laborious and time
consuming, we used the Bean pod mottle virus (BPMV)-based
vector for rapid and efficient protein expression and gene silencing.
The present study focuses on the functional roles of the gene
encoding the soybean CaM isoform GmCaM4. Overexpression of
GmCaM4 in soybean resulted in enhanced resistance to three
plant pathogens and increased tolerance to high salt conditions.
To gain an understanding of the underlying mechanisms, we
examined the potential defence pathways involved. Our studies
revealed activation/increased expression levels of pathogenesisrelated
(PR) genes in GmCaM4-overexpressing plants and the
accumulation of jasmonic acid (JA). Silencing of GmCaM4,
however, markedly repressed the expression of PR genes.We confirmed
the in vivo interaction between GmCaM4 and the CaM
binding transcription factor Myb2, which regulates the expression
of salt-responsive genes, using the yeast two-hybrid (Y2H) system
and bimolecular fluorescence complementation assays. GmCaM4
and Glycine max CaM binding receptor-like kinase (GmCBRLK) did
not interact in the Y2H assays, but the interaction between
GmCaM2 and GmCBRLK was confirmed. Thus, a GmCaM2–
GmCBRLK-mediated salt tolerance mechanism, similar to that
reported in Glycine soja, may also be functional in soybean. Confocal
microscopy showed subcellular localization of the green
fluorescent protein (GFP)-GmCaM4 fusion protein in the nucleus
and cytoplasm.
Not Available
 
Date 2018-12-17T08:29:04Z
2018-12-17T08:29:04Z
2014
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/16033
 
Language English
 
Relation Not Available;
 
Publisher BSPP