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Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

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Title Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion
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Creator A. Mann, R. P. Yadav, J. Singh, D. Kumar, B. Singh and P. S. Yadav
 
Subject Amnion cells, Buffalo, Differentiation, Pluripotency, Stem cells
 
Description Not Available
Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnionderived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesodermlineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine
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Date 2018-09-11T07:06:53Z
2018-09-11T07:06:53Z
2013-07-01
 
Type Research Paper
 
Identifier Mann A, R. P. Yadav, J. Singh, D. Kumar, B. Singh and P. S. Yadav. (2013) Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion. Cytotechnology. 65(1):23-30
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http://krishi.icar.gov.in/jspui/handle/123456789/6684
 
Language English
 
Relation Not Available;
 
Publisher Springer