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Characterization of bacteria degrading 3-hydroxy palmitic acid methyl ester (3OH-PAME), a quorum sensing molecule of Ralstonia solanacearum.

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Title Characterization of bacteria degrading 3-hydroxy palmitic acid methyl ester (3OH-PAME), a quorum sensing molecule of Ralstonia solanacearum.
Not Available
 
Creator Achari, G.A. and Ramesh, R
 
Subject 3OH-PAME, endophytic bacteria, quorum quenching, Ralstonia solanacearum, xylem.
 
Description Not Available
Bacterial wilt pathogen Ralstonia solanacearum causes severe crop loss of
eggplant, which is of economic importance in India. 3-hydroxy palmitic acid
methyl ester (3OH-PAME) is the main quorum sensing molecule governing
the expression of virulence factors in R. solanacearum. Ability of 164 bacterial
isolates from the xylem of eggplant (Solanum melongena L.), chilli pepper
(Capsicum annuum L.) and wild eggplant (Solanum torvum Sw.) to degrade
3OH-PAME was tested by disc diffusion assay. Enzymatic degradation of 3OHPAME
by five bacteria was confirmed by High-Performance Liquid
Chromatography–Mass Spectrometry analysis. 3OH-PAME degrading bacteria
were identified as Stenotrophomonas maltophilia, Pseudomonas aeruginosa and
Rhodococcus corynebacterioides. 3OH-PAME degrading bacteria reduced the
expression of virulence factors (exopolysaccharides and endoglucanase) of
R. solanacearum in vitro and reduced wilt incidence in eggplant seedlings under
greenhouse conditions. Isolates with quorum quenching activity successfully
re-colonized eggplant seedlings. Quorum quenching bacteria produced
antagonistic compounds, which may act synergistically with quorum quenching
in reducing bacterial wilt in eggplant.
Indian Council of Agricultural Research (ICAR), New Delhi, through ‘Outreach project on Phytopthora, Fusarium and Ralstonia diseases of horticultural and field crops’- (PhytoFuRa)
 
Date 2018-11-09T12:20:16Z
2018-11-09T12:20:16Z
2015-01-05
 
Type Research Paper
 
Identifier Achari, G.A. and Ramesh, R. 2015. Characterization of bacteria degrading 3-hydroxy palmitic acid methyl ester (3OH-PAME), a quorum sensing molecule of Ralstonia solanacearum. Letters in Applied Microbiology. 60 (5): 447-455.
1472765X
http://krishi.icar.gov.in/jspui/handle/123456789/10139
 
Language English
 
Relation Not Available;
 
Publisher Wiley Online Library