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Genetic diversity of Ralstonia solanacearum infecting solanaceous vegetables from India reveals the existence of unknown or newer sequevars of Phylotype I strains.

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Title Genetic diversity of Ralstonia solanacearum infecting solanaceous vegetables from India reveals the existence of unknown or newer sequevars of Phylotype I strains.
Not Available
 
Creator Ramesh, R., Achari, G.A. and Gaitonde, S.
 
Subject Ralstonia solanacearum. Diversity. Bacterial wilt . India . Eggplant . Phylotype . Sequevar . egl . pga . hrpB
 
Description Not Available
Bacterial wilt is one of the important constraints
in the cultivation of solanaceous vegetables in
India. The disease is caused by Ralstonia solanacearum,
a soil bacterium. We have collected 232 isolates of
R. solanacearum infecting solanaceous vegetables (eggplant,
tomato and chilli) and other crops from different
parts of India. Pathogenicity of the isolateswas tested on
eggplant, tomato and chilli and the pathogen was confirmed
by PCR. Multiplex PCR and biochemical tests
indicated that all the isolates were phylotype I and
biovar 3. Ninety-five representative isolates selected
based on geographical region, host range and pathogenicity
were subjected to further phylogenetic and diversity
analysis. Sequence analysis of egl, pga and hrpB
genes of 95 isolates and genetic diversity of 50 representative
isolates was reported and discussed. Indian
isolates within the Phylotype I did not group based on
the host or geographical location, except clustering of
isolates from the Andaman Islands. Indian isolates clustered
into two sub groups based on egl and pga trees
indicating the presence of two major population groups.
Sub group 1 is the dominant group in the data set and
consists of unknown/newer sequevars, and sub group 2
consist of mainly the isolates which are designated with
sequevar numbers based on egl sequences. In the hrpB
based tree, the sub group 2 is the dominant group in the
data set and it is the same for the sub group 1 of the egl
tree. Indian phylotpe I R. solanacearum strains are phenotypically diverse including the previously described
sequevars 14, 17, 44, 47 and 48. Our studies
indicated the existence of R. solanacearum isolates with
unknown/newer sequevars; the diversity existing among
the phylotype I isolates might be due to a continuous
evolutionary process. To our knowledge this is the first
detailed report on the diversity of phylotype I
R. solanacearum strains infecting solanaceous vegetables
and the existence of unknown/newer sequevars in
India.
Indian Council of Agricultural Research (ICAR), New Delhi, through ‘Outreach project on Phytopthora, Fusarium and Ralstonia diseases of horticultural and field crops’- (PhytoFuRa)
 
Date 2018-11-09T12:21:19Z
2018-11-09T12:21:19Z
2014-08-13
 
Type Research Paper
 
Identifier Ramesh, R., Achari, G.A. and Gaitonde, S. 2014. Genetic diversity of Ralstonia solanacearum infecting solanaceous vegetables from India reveals the existence of unknown or newer sequevars of Phylotype I strains. European Journal of Plant Pathology. 140 (3): 543-562.
0929-1873
http://krishi.icar.gov.in/jspui/handle/123456789/10142
 
Language English
 
Relation Not Available;
 
Publisher Springer