Genetic characterization of Ralstonia solanacearum infecting eggplant Solanum melongena L. from Goa and Western region of India.
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Title |
Genetic characterization of Ralstonia solanacearum infecting eggplant Solanum melongena L. from Goa and Western region of India.
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Creator |
Gaitonde, S. and Ramesh, R.
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Subject |
Ralstonia solanacearum, eggplant, pathogenicity, BOX-PCR, ERIC-PCR
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Description |
Not Available
A systematic study was undertaken to study the genetic diversity of R. solanacearum infecting solanaceous vegetables from Western regions of India. Fifty isolates of R. solanacearum were studied for their host range and were characterized using BOX and ERIC PCR. Out of these, 33 were isolated from eggplant, chilli, tomato and others were obtained from culture collection of Plant Pathology Laboratory, ICAR Research Complex for Goa. Pathogenicity of these isolates tested on three different hosts indicated that 49 isolates were pathogenic on eggplant, 44 isolates on tomato and 11 isolates on chilli. Results indicated that all the isolates studied belonged to Phylotype I based on the ITS region and biovar 3, except one isolate which is grouped under biovar 6 as per new classification. However, BOX and ERIC fingerprinting technique showed wide genetic variation among the isolates exhibiting 15 and 14 unique fingerprint patterns and 9 and 14 clusters respectively. This indicates that though R. solanacearum from Goa are phenotypically similar, great variation exists when whole genome is analyzed by rep-PCR. Indian Council of Agricultural Research (ICAR), New Delhi, through ‘Outreach project on Phytopthora, Fusarium and Ralstonia diseases of horticultural and field crops’- (PhytoFuRa) |
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Date |
2018-11-09T12:20:47Z
2018-11-09T12:20:47Z 2014-07-28 |
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Type |
Research Paper
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Identifier |
Gaitonde, S. and Ramesh, R. 2014. Genetic characterization of Ralstonia solanacearum infecting eggplant Solanum melongena L. from Goa and Western region of India. International Journal of Current Science. 12: E128-139.
2250-1770 http://krishi.icar.gov.in/jspui/handle/123456789/10140 |
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Language |
English
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Relation |
Not Available;
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Publisher |
IJCS New Liberty Group
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