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Degradation of Sulphonated Azo Dye Red HE7B by Bacillus sp. and Elucidation of Degradative Pathways

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Title Degradation of Sulphonated Azo Dye Red HE7B by Bacillus sp. and Elucidation of Degradative Pathways
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Creator Jyoti Kumar Thakur • Sangeeta Paul • Prem Dureja • K. Annapurna • Jasdeep C. Padaria • Madhuban Gopal
 
Subject Azo Dye, Red HE7B, Bacillus, Degradative Pathways
 
Description Not Available
Abstract Bacteria capable of degrading the sulfonated
azo dye Red HE7B were isolated from textile mill effluent
contaminated soil. The most efficient isolate was identified
as Bacillus sp. Azo1 and the isolate could successfully
decolorize up to 89 % of the dye. The decolorized cultural
extract analyzed by HPLC confirmed degradation. Enzymatic
analysis showed twofold and fourfold increase in the
activity of azoreductase and laccase enzymes, respectively,
indicating involvement of both reductive and oxidative
enzymes in biodegradation of Red HE7B. Degraded products
which were identified by GC/MS analysis included
various metabolites like 8-nitroso 1-naphthol, 2-diazonium
naphthalene. Mono azo dye intermediate was initially
generated from the parent molecule. This mono azo dye
was further degraded by the organism, into additional
products, depending on the site of cleavage of R–N=N–R
molecule. Based on the degradation products identified,
three different pathways have been proposed. The mechanism
of degradation in two of these pathways is different
from that of the previously reported pathway for azo dye
degradation. This is the first report of a microbial isolate
following multiple pathways for azo dye degradation. Azo
dye Red HE7B was observed to be phytotoxic, leading to
decrease in root development, shoot length and seedling
fresh weight. However, after biotreatment the resulting
degradation products were non-phytotoxic.
Not Available
 
Date 2019-11-27T09:56:38Z
2019-11-27T09:56:38Z
2014-03-30
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/25655
 
Language English
 
Relation Not Available;
 
Publisher Not Available