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RAPD and Nuclear rDNA ITS Polymorphism Within Macrophomina phaseolina Isolated from Arid Legumes of Western Rajasthan

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Title RAPD and Nuclear rDNA ITS Polymorphism Within Macrophomina phaseolina Isolated from Arid Legumes of Western Rajasthan
Not Available
 
Creator Ragini Gautam
S. K. Singh
Vinay Sharma
 
Subject R. bataticola
M. phaseolina
Genetic diversity
RAPD
rDNA analysis
 
Description Not Available
Concurrent heat and moisture stress often
favours root diseases caused by Macrophomina phaseolina
in arid legumes. Molecular analysis revealed 92 % variation
within M. phaseolina populations as compared to 8 %
among populations. The first three principle coordinates of
PCA analysis accounted for a 69.61 % of total variance and
Eigen vectors revealed 22.89 % of total variability. The
mean values of all the four populations together for Nei’s
gene diversity (h) was 0.1990 and Shannon’s information
index (i) was 0.3113. The result showed that the genetic
diversity of M. phaseolina isolates of population 2 (cowpea)
was richest among all the four populations. Analysis
of molecular variance indicated that main proportion of
genetic variation was within the host than among different
hosts. Out of 13 representative isolates seven were
molecularly identified as Rhizoctonia bataticola and six as
M. phaseolina upon sequencing of 5.8S RNA region.
Besides length variation in ITS-1, 5.8S rRNA gene, ITS-2
and total length, the authors report insertion/deletions at a
number of places in 13 isolates sequenced. This study
underlines that M. phaseolina distribution is independent of
host and/or geography and validates the utility of ITS
rDNA region as a reliable indicator of phylogenetic interrelationships
as an additional approach for identification of
the genus Macrophomina and assessing its genetic
diversity.
Not Available
 
Date 2019-05-14T11:52:11Z
2019-05-14T11:52:11Z
2014-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19483
 
Language English
 
Relation Not Available;
 
Publisher Springer