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Occurrence and characterization of hitherto unknown Streptomyces species in semi-arid soils

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Title Occurrence and characterization of hitherto unknown Streptomyces species in semi-arid soils
Not Available
 
Creator Surendra Kumar
E. Priya,
Dilip Singh Solanki
Ruchika Sharma
Praveen Gehlot
Rakesh Pathak
S.K. Singh
 
Subject 16SrRNA gene
Diversity
GC content
Streptomyces
 
Description Not Available
Streptomyces the predominant genus of Actinobacteria and plays an important role in the recycling of soil organic matter and production of important secondary metabolites. The occurrence and diversity assessment of species revealed alkaline and poor nutrient status of soils of semi-arid region of Jodhpur, Rajasthan. The morphological and biochemical characterization of 21 isolates facilitated Genus level identification but were insufficient to designate species. Species designation based on 16S rRNA gene delineated 21 isolates into 14 species. Upon BLAST search, the test isolates exhibited 98 to 100% identities with that of the best aligned sequences of the NCBI database. The GC content of 16S rRNA gene sequences of all the Streptomyces isolates tested ranged from 59.03% to 60.94%. The
multiple sequence alignment of all the 21 isolates generated a phylogram with high
bootstrap values indicating reliable grouping of isolates based on nucleotide sequence variations
by way of insertion, deletion and substitutions and 16S rRNA length polymorphism. Some of the
Streptomyces species molecularly identified under present study are reported for the first time
from semi-arid region of Jodhpur.
Not Available
 
Date 2019-05-14T11:15:34Z
2019-05-14T11:15:34Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19463
 
Language English
 
Relation Not Available;
 
Publisher Triveni Enterprises Lucknow, India