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Assessment of genetic diversity in clusterbean using nuclear rDNA and RAPD markers

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Title Assessment of genetic diversity in clusterbean using nuclear rDNA and RAPD markers
Not Available
 
Creator RAKESH PATHAK
S.K. SINGH
MANJIT SINGH
 
Subject Clusterbean
Cyamopsis tetragonoloba
Genetic diversity
ITS sequencing
RAPD
SNP
 
Description Not Available
The extent of genetic variability among 18 genotypes of
clusterbean using genetic markers was assessed. Multi-locus
genotyping by 9 RAPD primers detected 87.6% intra-specific
polymorphism. UPGMA dendrogram clearly delineated the
genotypes into four clusters irrespective of their place of origin.
Analysis of molecular variance indicated that a greater
proportion of total genetic variation exists within population
rather than among populations. PCR-based amplification of
ITS regions encompassing 5.8S rRNA gene yielded a single
band of uniform length of 618 bp in all the samples sequenced.
However, multiple sequence alignment of ITS regions of
representative genotypes belonging to major RAPD clusters
exhibited single nucleotide polymorphism. Thus, these two
marker systems validated the existence of genetic variation in
studied clusterbean genotypes which can be used in crop
improvement.
Not Available
 
Date 2019-05-15T12:02:16Z
2019-05-15T12:02:16Z
2011-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19565
 
Language English
 
Relation Not Available;
 
Publisher Not Available