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Studies on genetic divergence among Indian varieties of a spice herb Coriandrum sativum

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Title Studies on genetic divergence among Indian varieties of a spice herb Coriandrum sativum
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Creator S.K. Singh
R.K. Kakani
R.S. Meena
Anjly Pancholy
Rakesh Pathak
Aparna Raturi
 
Subject Coriandrum sativum
Coriander
Genetic diversity
RAPD
rDNA diversity
DNA bar coding
 
Description Not Available
Coriander (Coriandrum sativum L.) is an annual spice herb that belongs to umbel family Apiaceae with diversified uses. We investigated the extent of variability among 22 Indian varieties of coriander using phenotypic and genetic markers. Multilocus genotyping by nine RAPD primers detected an average of intraspecific variations amounting to 66.18 % polymorphism in banding patterns. Analysis of molecular variance indicated that a greater proportion of total genetic variation exists within population (98%) rather than among populations (2%). Higher values of Nei’s gene diversity (h) and Shannon Information Index (i) and genetic distance analysis validate wider genetic diversity among Indian coriander varieties. Besides total internal transcribed spacer (ITS) length variations and single nucleotide polymorphisms, insertions/deletions (INDELS) were detected at seven sites in ITS-1 region. Multiple sequence alignment of 12 sequenced varieties revealed cent per cent identities of 5.8S gene region (162 bp) that validates its conserved nature. Multiple sequence alignment of ITS-1 region may be of phylogenetic significance in distinguishing and cataloguing of coriander germplasm. The representative sequences of each subgroup and all distinct varieties of RAPD clusters have been submitted to NCBI database and assigned Gen Accession numbers HQ 377194-377205. The measures of relative genetic distances among the varieties of coriander did not completely correlate the geographical places of their development. Eventually, the knowledge of their genetic relationships and DNA bar coding will be of significance.
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Date 2019-05-15T08:45:06Z
2019-05-15T08:45:06Z
1001-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/19522
 
Language English
 
Relation Not Available;
 
Publisher Not Available