Record Details

Molecular Markers Assisted Identification of Intraspecific Hybrids in Ziziphus mauritiana

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular Markers Assisted Identification of Intraspecific Hybrids in Ziziphus mauritiana
Not Available
 
Creator Sunil Kumar Singh
P. R. Meghwal
Rakesh Pathak
Ragini Gautam
 
Subject Indian jujube
Seb
Tikadi
Katha
Hybridization
ITS
RAPD
 
Description Not Available
Breeding in Ziziphus mauritiana through
hybridization is limited by its small sized flowers, cross
incompatibility, low fruit set and poor retention. Correct
identification of a hybrid is often difficult when it resembles
more with one parent or when new morphological
combination of characters arise from recombination of
distinct genotypes. Under present study three varieties of
Z. mauritiana viz., Tikadi, Seb and Katha were found cross
compatible and resulted in improved hybrids viz., F1
(Seb 9 Tikadi), F1 9 Seb and Seb 9 Katha. Ten RAPD
primers revealed a total of 108 bands in the range of 150 bp
to 3 kb. Among these, 69 bands were polymorphic and
exhibited 33.33–90.9 % polymorphism in the banding
patterns. The dendrogram clearly delineated variety Tikadi
and Katha from Seb. All the three hybrids formed a single
cluster with the variety Seb. The 5.8S gene region was
found to be highly conserved (99.39 %) followed by ITS-2
(97.77 %). and ITS-1 (90.6–90.8 %). Heterozygous positions
in ITS sequences clearly show their hybrid nature and
could perform as highly polymorphic molecular markers.
Despite SNPs, INDELS and total length polymorphism
among parents and their hybrids, all were molecularly
identified as Z. mauritiana and did not cause any phylogenetic
error. The nucleotide polymorphism in ITS-1
region can serve as a bar code to detect and address genetic
diversity within Z. mauritiana.
Not Available
 
Date 2019-05-15T08:48:58Z
2019-05-15T08:48:58Z
1001-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19525
 
Language English
 
Relation Not Available;
 
Publisher Not Available