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Purification and characterization of a novel thermostable antifungal protein with chitinase activity from mung bean

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Title Purification and characterization of a novel thermostable antifungal protein with chitinase activity from mung bean
Not Available
 
Creator D.S. Solanki
S. Kumar
K. Parihar
K. Sharma
P. Gehlot
S.K. Singh
R. Pathak
 
Subject Antifungal protein Chitinase activity Chromatography SDS-PAGE Thermostability
 
Description Not Available
Antifungal proteins are produced by many plant species and participate in defence mechanisms
against number of fungal pathogens. The main objective of the study was to purify and characterize the
thermo-stable antifungal compound with chitinase activity from mung bean seeds to assess their antifungal potency. The antifungal protein was isolated through ammonium sulphate precipitation method followed by its purification through ion-exchange chromatography. The purified protein was characterized by evaluating its antifungal efficacy, thermal stability, chitinase activity andSDSPAGEprofiling. Molecular mass of characterized antifungal protein was 50.6 kDa. Purified protein exhibited antifungal activity against pathogenic fungi and and
sustained its thermo-stability up to 60°C with chitinase activity. The isolated antifungal proteins showed unique column chromatographic behaviour, molecular weight, specificity of chitinase activity and relatively high thermo-stability with potent antifungal activity. It can be used in different biomedical and pharmaceutical applications as bio-pesticides. Vigna radiata Macrophomina phaseolina Magnaporthe grisea Characterization of antifungal protein
Not Available
 
Date 2019-05-15T11:58:31Z
2019-05-15T11:58:31Z
2018-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19561
 
Language English
 
Relation Not Available;
 
Publisher Not Available