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Genetic characterization of plant growth promoting rhizobacteria of two prominent leguminous trees in western Rajasthan

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Title Genetic characterization of plant growth promoting rhizobacteria of two prominent leguminous trees in western Rajasthan
Not Available
 
Creator Anjly Pancholy
S.K. Singh
S.K. Jindal
Rakesh Pathak
 
Subject Acacia senegal
Prosopis cineraria
Polymorphism
16S rRNA
 
Description Not Available
Plant growth promoting rhizobacteria (PGPRs) from the rhizosphere and root nodules of native Acacia senegal (L.) Willd. and Prosopis cineraria (L.) Druce trees in Western Rajasthan were molecularly characterized by direct sequencing of the 16S rRNA gene. Of the nine isolates obtained from A. senegal, the lone rhizospheric isolate was identified as Stephyllococus hominis Kloos and Schleifer and the remaining eight from root nodules were characterised as Sinorhizobium saheli De Lajudie. Eight isolations from P. cineraria included Lysinibacillus sphaericus Meyer Neide and Bacillus subtilis (Ehrenberg) Cohn (one isolate each) from rhizosphere and Sinorhizobium kostiense Nick (one) and S. saheli (five) from root nodules. Eight RAPD primers detected
intra-specific variations generating scorable amplicons and revealed 130 bands with 100% polymorphism. The phylogram
based on multiple sequence alignment of 16S rRNA gene delineated not only different genera of PGPRs but also recognized
four strains of S. saheli.
Not Available
 
Date 2019-05-15T12:00:21Z
2019-05-15T12:00:21Z
2011-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19563
 
Language English
 
Relation Not Available;
 
Publisher Not Available