Record Details

Novel and conserved heat-responsive microRNAs in wheat (Triticum aestivum L.)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Novel and conserved heat-responsive microRNAs in wheat (Triticum aestivum L.)
Not Available
 
Creator Ranjeet Ranjan Kumar
Himanshu Pathak
Sushil Kumar Sharma
Yugal Kishore Kala
Mahesh Kumar
Gyanendra Pratap Singh
Suneha Goswami
Raj Deo Rai
 
Subject Heat stress
miRNA
Ilumina HiSeq
 
Description Not Available
MicroRNAs (miRNAs) are small endogenous
RNAs of ~22 nucleotides that have been shown to play
regulatory role by negatively affecting the expression of genes
at the post-transcriptional level. Information of miRNAs on
some important crops like soybean, Arabidopsis, and rice, etc.
are available, but no study on heat-responsive novel miRNAs
has yet been reported in wheat (Triticum aestivum L.). In the
present investigation, a popular wheat cultivar HD2985 was
used in small RNA library construction and Illumina HiSeq
2000 was used to perform high-throughput sequencing of the
library after cluster generation; 110,896,604 and 87,743,861
reads were generated in the control (22 °C) and heat-treated
(42 °C for 2 h) samples, respectively. Forty-four precursor and
mature miRNAs were found in T. aestivum from miRBase v
19. The frequencies of the miRNA families varied from 2 (taemiR1117)
to 60,672 (tae-miR159b). We identify 1052 and
902 mature miRNA sequences in HD2985 control and HStreated
samples by mapping on reference draft genome of
T. aestivum. Maximum identified miRNAs were located on
IWGSC_CSS_3B_scaff (chromosome 3B).We could identify
53 and 46 mature miRNA in the control and HS samples and
more than 516 target genes by mapping on the reference
genome of Oryza sativa, Zea mays, and Sorghum bicolor.
Using different pipelines and plant-specific criteria, 37 novel
miRNAs were identified in the control and treated samples.
Six novel miRNA were validated using qRT-PCR to be heatresponsive.
A negative correlation was, however, observed
between the expression of novel miRNAs and their targets.
Target prediction and pathway analysis revealed their involvement
in the heat stress tolerance. These novel miRNAs are
new additions to miRNA database of wheat, and the regulatory
network will be made use of in deciphering the mechanism
of thermotolerance in wheat
Not Available
 
Date 2019-07-03T09:11:05Z
2019-07-03T09:11:05Z
2015-05-01
 
Type Research Paper
 
Identifier 53
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/21095
 
Language English
 
Relation Not Available;
 
Publisher Springer Berlin Heidelberg