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Arbitrary Primer Based RAPD- A Useful Genetic Marker for Species Identification in Morels

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Title Arbitrary Primer Based RAPD- A Useful Genetic Marker for Species Identification in Morels
Not Available
 
Creator Singh, S.K.
Kamal, Shwet
Tiwari, M.
Yadav, M.C.
Upadhyay. R.C.
 
Subject Morchellaceae, Morchella, Verpa conica, Mitrophora semilibera, RAPD, 5.8S r RNA gene, Phylogeny.
 
Description Not Available
Internal transcribed spacer (ITS) regions of 5.8S ribosomal DNA gene of 46 monospore cultures representing different species of family Morchellaceae were sequenced. Eight putative species namely Morchella esculenta, M. crassipes, M. angusticeps, M. conica, Mitrophora semilibera, Morchella spongiola, M. vulgaris and Verpa conica were designated to the sequenced cultures analyzed based on similarity with the best aligned sequence of the BLAST search. We recorded interspecific polymorphism in ITS regions of the 5.8S r-DNA gene amongst putative species of morels. However no intraspecific ITS polymorphism could be visualized amongst monospores from single and or different ascocarp collected from various geographical regions. PCR RAPD amplified profiles of different monospores were identical at intraspecific levels and could not be distinguished by eight arbitrary primers tested. RAPD profiles exhibited significant interspecific polymorphism distinguishing all the eight putative species from each other by primer OPP-6 (5'- GTG GGT TGA C- 3') and a custom primer (5'-CGC ACC GCA G-3'). RAPD profiles generated using aforesaid arbitrary primers can serve as useful genetic markers for species identification and to improve morel systematics. Present study is the first report on exploitation of random primers in species differentiation in morels.
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Date 2019-07-18T11:24:02Z
2019-07-18T11:24:02Z
2004-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/21518
 
Language English
 
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Publisher Not Available