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. Morel Phylogeny and diagnostics based on restriction fragment length polymorphism analysis of ITS region of 5.8S ribosomal DNA

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Title . Morel Phylogeny and diagnostics based on restriction fragment length polymorphism analysis of ITS region of 5.8S ribosomal DNA
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Creator Singh, S.K.
Tiwari, M.
Kamal, Shwet
Yadav, M.C.
 
Subject Morchellaceae, Morchella, Verpa conica, Mitrophora semilibera, RFLP, ARDRA, 5.8S rRNA gene, phylogeny.
 
Description Not Available
Thirty-Six cultures representing eight Morchella and related genera, namely, Morchella esculenta, M. crassipes, M. spongiola, M. vulgaris, M. angusticeps, M. conica, Mitrophora semilibera and Verpa conica were subjected to restriction analysis of ITS1-5.8S-ITS2 region of rDNA. Six restriction endonuclease enzymes viz. Taq 1, Eco R1, Msp 1, Rsa 1, Hin f1 and Bsu R1 were used to generate restriction fragments and analysis of phylogenetic relationships amongst morels. The Amplified Ribosomal DNA Restriction Analysis (ARDRA) not only distinguished yellow morels from black morels but also separated related genera Mitrophora semilibera and Verpa conica from true morels. Simultaneously, each morel species could be separated from each other exhibiting considerable phylogenetic distances. The unique restriction fragment profiles generated by the restriction endonucleases enabled us to identify marker fragments to distinguish each species within and amongst the morel group. Since no intra-specific variation in restriction profiles by the six restriction endonucleases could be visualized among monospores, the technique could be used for rapid identification of wild morel specimens as a cheap alternative to direct sequencing for germplasm cataloguing.
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Date 2019-07-18T11:24:56Z
2019-07-18T11:24:56Z
2005-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/21519
 
Language English
 
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Publisher Not Available