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Molecular Identifi cation of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data

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Title Molecular Identifi cation of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data
Not Available
 
Creator Singh, S.K.
Rai, R.D.
Kamal, Shwet
 
Subject Aphyllophorales, Ganoderma, Trametes, Tyromyces, gene sequencing, 5.8S rRNA gene
 
Description Not Available
Analysis of genetic relationship of important Aphyllophorales germplasm accessions of
the National Research Centre for Mushroom, Solan, India, was conducted using RAPD and ITS rDNA
sequences. RAPD profi les exhibited signifi cant variations among Trametes versicolor, Tyromyces chioneus,
and Ganoderma species. Th e resulting RAPD-based phylogram grouped Ganoderma accessions into
four clades. Phylogeny inferred from ITS rDNA sequence distinguished Trametes versicolor, Tyromyces
chioneus, and Ganoderma sp. at the generic level due to diff erent lengths and variations of the 5.8S rDNA
conserved region. At the intrageneric level, Ganoderma accessions were divided into two distinct groups
based on nucleotide substitution in ITS-1 and ITS-2 regions. It is presumed that some of the Ganoderma
sp. samples might be G. lucidum. Single nucleotide substitutions turned out to be more useful for genetic
cataloguing of isolates within a species than sequence alignment using NCBI databse.
Not Available
 
Date 2019-07-18T11:27:57Z
2019-07-18T11:27:57Z
2005-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/21522
 
Language English
 
Relation Not Available;
 
Publisher Not Available