Record Details

An integrated system for simultaneous casting of multi-polyacrylamide gels with varied concentrations

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title An integrated system for simultaneous casting of multi-polyacrylamide gels with varied concentrations
Not Available
 
Creator Praveen Maurye, Mrigank Dhabi, Jayanta Kumar Biswas, Tapas Kumar Bandyopadhyay
 
Subject Casting apparatusMultiple polyacrylamide gelsProteinElectrophoresis
 
Description Not Available
The ever growing advancements in the protein analysis of any cell or tissue using different variants of polyacrylamide gel electrophoresis and associated basic techniques offers its widespread application in almost all molecular biology laboratories. On the other hand, casting of several gels in parallel is a cumbersome task but is often an essential situation. A multi-gel casting apparatus for simultaneous casting of homogeneous concentration and gradient concentration sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) gel was described here. The efficiency of this newly modified apparatus was significantly improved by the flexibility of using the top pouring and bottom pouring mechanism at the same time along with the siphoning of used solution from polymerizing bottom gel surface which makes it suitable for all types of vertical polyacrylamide gels. This apparatus reliably produces different kinds of slab gels in multiple numbers with each gel having similar physical characteristics in its type and provides desired results comparable to the gels made by commercial sources.
ICAR
 
Date 2018-10-04T02:48:00Z
2018-10-04T02:48:00Z
2018-01-01
 
Type Article
 
Identifier An integrated system for simultaneous casting of multi-polyacrylamide gels with varied concentrations P Maurye, M Dhabi, JK Biswas, TK Bandyopadhyay - Measurement, 2018
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/7454
 
Language English
 
Relation Not Available;
 
Publisher Elsevier