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Isolation and molecular characterization of plant growth-promoting Bacillus spp. and their impact on sugarcane (Saccharum spp. hybrids) growth and tolerance towards drought stress

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Title Isolation and molecular characterization of plant growth-promoting Bacillus spp. and their impact on sugarcane (Saccharum spp. hybrids) growth and tolerance towards drought stress
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Creator Priyanka Chandra
Pramila Tripathi
Amaresh Chandra
 
Subject Plant growth-promoting rhizobacteria · Sugarcane · Antioxidant enzyme system · Bacillus megaterium · Bacillus subtilis · qRT-PCR · RT-PCR
 
Description Not Available
Plant growth-promoting rhizobacteria (PGPR) have demonstrated its importance in agriculture globally including beneficial
dynamics change in plant rhizosphere leading better tolerance towards abiotic stresses. Hundred and one bacterial cultures
from sugarcane rhizosphere zone of > 50 years of sugarcane growing fields were isolated using standard protocols and were
further subjected to in vitro screening to visualize their impact on plant growth. Of these, two cultures based on biochemical
test and 16S rRNA gene sequences were classified as Bacillus subtilis (BSSC11) and Bacillus megaterium (BMSE7).
Sugarcane settlings exposed to these strains exhibited more nutrient content, improved growth in terms of early sprouting,
increased vigor (high shoot and root weight) and better antioxidant enzyme system ability including quantitative overexpression
of superoxide dismutase (SOD) isoforms over controls. Treated cane seed (setts) with B. megaterium culture exhibited
high expression of invertase genes which facilitated early and improved growth of settlings through increased inversion of
sucrose to glucose and fructose. When these settlings were exposed to drought, a significant decrease in SOD enzyme activity
and increase in proline content was observed especially in B. megaterium-exposed samples indicating less generation of free
radicals in inoculated than those of non-inoculated samples where SOD activity increased significantly. This is apparently
a first study of PGPRs isolated from continuous growing sugarcane fields on the growth and vigor of sugarcane settlings
in vivo and further hypothesized that a multiple chain of events is involved in imparting better crop growth of PGPR-exposed
settlings both under normal and stress conditions.
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Date 2019-10-31T05:40:58Z
2019-10-31T05:40:58Z
2018-10-26
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/24226
 
Language English
 
Relation Not Available;
 
Publisher Not Available