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Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis

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Title Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis
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Creator Mishra P, Ajay Jain, Teruhiro Takabe, Yoshito Tanaka, Manisha Negi, Nisha Singh, Neha Jain, Vagish Mishra, R Maniraj, S L Krishnamurthy, Rohini Sreevathsa, Nagendra Kumar Singh, Vandna Rai.
 
Subject rice salinity
 
Description Not Available
Among abiotic stresses, salt stress adversely affects growth and development in rice.
Contrasting salt tolerant (CSR27), and salt sensitive (MI48) rice varieties provided
information on an array of genes that may contribute for salt tolerance of rice. Earlier
studies on transcriptome and proteome profiling led to the identification of salt stressinduced
serine hydroxymethyltransferase-3 (SHMT3) gene. In the present study, the
SHMT3 gene was isolated from salt-tolerant (CSR27) rice. OsSHMT3 exhibited salinitystress
induced accentuated and differential expression levels in different tissues of rice.
OsSHMT3 was overexpressed in Escherichia coli and assayed for enzymatic activity
and modeling protein structure. Further, Arabidopsis transgenic plants overexpressing
OsSHMT3 exhibited tolerance toward salt stress. Comparative analyses of OsSHMT3
vis a vis wild type by ionomic, transcriptomic, and metabolic profiling, protein expression
and analysis of various traits revealed a pivotal role of OsSHMT3 in conferring tolerance
toward salt stress. The gene can further be used in developing gene-based markers for
salt stress to be employed in marker assisted breeding programs.
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Date 2019-12-04T04:01:06Z
2019-12-04T04:01:06Z
2019-01-01
 
Type Research Paper
 
Identifier 8. Mishra P, Ajay Jain, Teruhiro Takabe, Yoshito Tanaka, Manisha Negi, Nisha Singh, Neha Jain, Vagish Mishra, R Maniraj, Krishnamurthy S. L., Rohini Sreevathsa, Nagendra Kumar Singh, Vandna Rai. 2019. Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis. Frontiers in Plant Science. doi: 10.3389/fpls.2019.00217.
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http://krishi.icar.gov.in/jspui/handle/123456789/26801
 
Language English
 
Relation Not Available;
 
Publisher Frontiers in Plant Science