Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis
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Title |
Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis
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Creator |
Mishra P, Ajay Jain, Teruhiro Takabe, Yoshito Tanaka, Manisha Negi, Nisha Singh, Neha Jain, Vagish Mishra, R Maniraj, S L Krishnamurthy, Rohini Sreevathsa, Nagendra Kumar Singh, Vandna Rai.
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Subject |
rice salinity
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Description |
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Among abiotic stresses, salt stress adversely affects growth and development in rice. Contrasting salt tolerant (CSR27), and salt sensitive (MI48) rice varieties provided information on an array of genes that may contribute for salt tolerance of rice. Earlier studies on transcriptome and proteome profiling led to the identification of salt stressinduced serine hydroxymethyltransferase-3 (SHMT3) gene. In the present study, the SHMT3 gene was isolated from salt-tolerant (CSR27) rice. OsSHMT3 exhibited salinitystress induced accentuated and differential expression levels in different tissues of rice. OsSHMT3 was overexpressed in Escherichia coli and assayed for enzymatic activity and modeling protein structure. Further, Arabidopsis transgenic plants overexpressing OsSHMT3 exhibited tolerance toward salt stress. Comparative analyses of OsSHMT3 vis a vis wild type by ionomic, transcriptomic, and metabolic profiling, protein expression and analysis of various traits revealed a pivotal role of OsSHMT3 in conferring tolerance toward salt stress. The gene can further be used in developing gene-based markers for salt stress to be employed in marker assisted breeding programs. Not Available |
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Date |
2019-12-04T04:01:06Z
2019-12-04T04:01:06Z 2019-01-01 |
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Type |
Research Paper
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Identifier |
8. Mishra P, Ajay Jain, Teruhiro Takabe, Yoshito Tanaka, Manisha Negi, Nisha Singh, Neha Jain, Vagish Mishra, R Maniraj, Krishnamurthy S. L., Rohini Sreevathsa, Nagendra Kumar Singh, Vandna Rai. 2019. Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis. Frontiers in Plant Science. doi: 10.3389/fpls.2019.00217.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/26801 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Frontiers in Plant Science
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