Record Details

Impacts of acute toxicity of arsenic (III) alone and with high temperature on stress biomarkers, immunological status and cellular metabolism in fish

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Impacts of acute toxicity of arsenic (III) alone and with high temperature on stress biomarkers, immunological status and cellular metabolism in fish
Not Available
 
Creator Neeraj Kumar*, Gupta S, Bhushan S, Narendra Pratap Singh
 
Subject Arsenic Acetylcholine esterase Oxidative stress Immunological status temperature
 
Description Not Available
The water bodies are greatly influenced by heavy metal contamination and global increasing temperature.
Arsenic (As) is one of the most dangerous widespread pollutants that pose health threats to human, animals and
fishes. Considering the above, the study has been carried out to delineate 96 h median lethal concentration of
arsenic alone and in combination with high temperature (As-T, 34 °C) by conducting static non-renewable bioassay
acute toxicity in Pangasianodon hypophthalmus (average weight 6.25 ± 0.69 g, length 5.32 cm). Effect of
definitive doses such as 25, 26, 27, 28, 29 and 30 mg/L of As alone and in combination with high temperature
(As-T) were evaluated on stress biomarkers and cellular metabolism of P. hypophthalmus. The lethal concentration
(96 h LC50) of As alone and in combination with high temperature was found to be 28.16 mg/L and
26.88 mg/L, respectively. The stress biomarkers in terms of catalase, superoxide dismutase (SOD) and glutathione-
s-transferase (GST) in liver, gill, brain and kidney, blood glucose and NBT were remarkable higher
(p < 0.01) in comparison to unexposed group (control group). Brain neurotransmitter enzyme, AChE, immunological
status (blood glucose and NBT) and cellular metabolic enzymes (lactate dehydrogenase LDH,
malate dehydrogenase MDH, aspartate aminotransferase AST, and alanine aminotransferase ALT, glucose-6-
phosphate dehydrogenase G6PDH and ATPase) were noticeably (p < 0.01) altered by As and As-T exposure.
The histopathological study exhibited devastating changes with exposure to As and As-T such as bile stagnation,
hepatocyte with irregular nucleus, eosinophilic granules in the cytoplasm, necrosis, and nuclear hypertrophy in
liver and curling of secondary lamellae, hypertrophy of lamellar epithelium, blood congestion, incomplete fusion
of secondary lamellae, complete fusion of several lamellae and aneurysm in gill. Overall results clearly indicate
that acute exposure of As and high temperature led to pronounced deleterious alterations on stress biomarkers
and cellular and metabolic activities of P. hypophthalmus.
Not Available
 
Date 2019-12-05T07:07:26Z
2019-12-05T07:07:26Z
2019-09-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/27907
 
Language English
 
Relation Not Available;
 
Publisher Elsevierr