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Enhanced tolerance of transgenic sorghum expressing mtlD gene to water-deficit stress Indian Journal of Biotechnology

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Title Enhanced tolerance of transgenic sorghum expressing mtlD gene to water-deficit stress Indian Journal of Biotechnology
Enhanced tolerance of transgenic sorghum expressing mtlD gene to water-deficit stress Indian Journal of Biotechnology
 
Creator ICAR_CRIDA
 
Subject : mtlD, physiological evaluation, sorghum, water-deficit stress, yield .
 
Description Not Available
Transgenic sorghum (T4) lines with mannitol-1-phosphate dehydrogenase (mtlD) gene derived from Escherichia coli
were evaluated for water-deficit stress tolerance in pot culture under greenhouse conditions. Leaf water relations,
accumulation of total soluble sugars, membrane stability, antioxidative enzyme activity, yield and yield related parameters
were analyzed in the plants subjected to well-watered and water-deficit stress conditions. Water-deficit stress caused a
reduction in the soil moisture content resulting in visual symptoms of leaf rolling. Transgenics maintained relatively higher
relative water content (RWC) than the untransformed control 15 d after withholding water. The transgenics accumulated
more total soluble sugars when compared to untransformed control. Also the transgenics showed lower malondialdehyde
(MDA) content and increased superoxide dismutase (SOD) activity. All these traits together contributed to the better
performance of mtlD transgenics compared to untransformed control. Further, the percent decrease in total biomass and
grain yield under water-deficit stress was lower in transgenics compared to control. Most importantly the root biomass was
found to be significantly higher in the transgenics when compared to untransformed control. Among the transgenics, L2, L5
and L75 were found to be superior in terms of various physiological traits, such as, RWC, accumulation of total soluble
sugars and SOD activity related to drought tolerance compared to control and other mtlD transgenic lines, L3, L4 and L72.
Not Available
 
Date 2020-02-26T04:16:43Z
2020-02-26T04:16:43Z
2017-01
 
Type Technical Report
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/33128
 
Language English
 
Relation Not Available;
 
Publisher S K YADAV