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Molecular and Biochemical Approaches for Characterization of Antifungal Trait of a Potent Biocontrol Agent Bacillus subtilis RP24

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Title Molecular and Biochemical Approaches for Characterization of Antifungal Trait of a Potent Biocontrol Agent Bacillus subtilis RP24
Molecular and Biochemical Approaches for Characterization of Antifungal Trait of a Potent Biocontrol Agent Bacillus subtilis RP24
 
Creator ICAR_CRIDA
 
Subject Molecular, Biochemical Approaches, Characterization, Antifungal, Potent Biocontrol Agent, Bacillus subtilis RP24
 
Description Not Available
Bacillus subtilis strain RP24, isolated from
rhizoplane of field grown pigeon pea, exhibited in vitro
antagonism against a wide range of phytopathogenic fungi.
An attempt was made to partially purify and characterize
the diffusible antifungal metabolite/s produced by the
strain RP24 and its negative mutant (NM) in potato dextrose medium. High performance liquid chromatography
(HPLC) of partially purified extract of RP24 showed the
presence of lipopeptide antibiotic iturin as a major peak
that was comparable to that of standard iturin A
(5.230 min) from Sigma–Aldrich whereas the corresponding peak was absent in extract of NM. The structure was
further confirmed by liquid chromatographic mass spectrometric (LCMS) analysis as iturin A. LCMS analysis also
showed the presence of surfactin and fengycin besides
iturin A. Amplification of the lpa-14 (encodes the
40
-phosphopantetheinyl transferase required for the maturation of template enzyme of iturin A) and ituD (encodes a
putative malonyl coenzyme A transacylase, whose disruption results in a specific deficiency in iturin A production) genes of iturin operon of strain RP24 was carried out
and the sequences obtained were compared with the
existing database of NCBI. The sequences of lpa-14 and
ituD gene of RP24 showed 98% and 97% homology with
lpa-14 and ituD genes of B. subtilis in the existing database. The results indicated that strain RP24 harbors iturin
operon in its genome and a chemical mutation in this
operon might have resulted in loss of antifungal activity in
the negative mutant.
Not Available
 
Date 2020-02-25T09:23:19Z
2020-02-25T09:23:19Z
2010
 
Type Technical Report
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/32946
 
Language English
 
Relation Not Available;
 
Publisher Minakshi Grover, Lata Nain, Shashi Bala Singh, Anil Kumar Saxena