ICAR Research Data Repository for Knowledge Management
Efficient plant regeneration from shoot apices of sorghum
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Efficient plant regeneration from shoot apices of sorghum
Efficient regenration from shoot apices of sorghum ( Sorghum bicolor L. Moench) |
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| Creator |
ICAR_CRIDA
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| Subject |
2,4-dichlorophenoxyacetic acid, kinetin, plant growth regulators, Sorghum bicolor (L.) Moench.
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| Description |
Not Available
An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two cultivars of sorghum, SPV-462 and M35-1, as explants. A vertical slit given from the base of each dissected apex enhanced the efficiency of callusing response by two fold. MS medium containing 0.5 mg dm-3 each of 2,4-D and kinetin was most effective in producing friable and embryogenic calli. Scanning electron microscopy of these calli detected somatic embryogenesis. Calli thus induced gave rise to approximately 42 green shoots per callus in both the genotypes when transferred to regeneration medium containing 1.5 mg dm-3 kinetin. Not Available |
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| Date |
2020-02-25T05:28:53Z
2020-02-25T05:28:53Z 2006 |
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| Type |
Technical Report
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/32448 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
M. MAHESWARI, N. JYOTHI LAKSHMI, S.K. YADAV, M. VANAJA, B. VENKATESWARLU
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