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Hybridity Assessment in Experimental F1s of Rice (Oryza sativa L.) using Microsatellite Markers

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Title Hybridity Assessment in Experimental F1s of Rice (Oryza sativa L.) using Microsatellite Markers
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Creator Manohara KK, Morajkar S, Shanbagh Y, Phadte P, Patil K
 
Subject Coastal salinity, Heterozygosity, Landraces, Polymorphic markers
 
Description Not Available
The present investigation was undertaken to assess the hybridity of experimental F1 plants derived from
the crossing of salinity and/or submergence tolerant landraces with the high-yielding popular varieties of the west coast region of India. Seven microsatellite markers were utilized for confirming the hybridity of the 16 F1 plants developed from four cross combinations, viz. Karjat-3 X KS-19-2 (Cross 1), Goa dhan-3 X Jaddubatta (Cross 2), Jaya X KS-19-2 (Cross 3) and Karjat-3 X Jaddubatta (Cross 4). Polymorphism survey using above seven markers between the parents of all the four crosses revealed that six of them were polymorphic, whereas one marker was monomorphic. The marker RM21539 was found to be polymorphic in all the four cross combinations, followed by RM493, RM3412, RM206 and RM10843 exhibiting polymorphism in three crosses, while marker RM25181 was polymorphic in two crosses. The screening of F1s with polymorphic markers revealed that the F1s from Cross 3 exhibited heterozygosity (showing alleles from both the parents) at six marker loci, whereas the F1s from Cross 1 and Cross 4 at five loci and Cross 2 at two marker loci, thereby confirming hybridity in all the 16 tested F1 plants.
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Date 2020-06-20T10:10:30Z
2020-06-20T10:10:30Z
2019-01-02
 
Type Research Paper
 
Identifier Manohara KK, Morajkar S, Shanbagh Y, Phadte P, Patil K (2019) Hybridity assessment in experimental F1s of rice (Oryza sativa L.) using microsatellite markers. Natl. Acad. Sci. Lett. https://doi.org/10.1007/s40009-019-00832-y.
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http://krishi.icar.gov.in/jspui/handle/123456789/37325
 
Language English
 
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