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Characterization of Nanoclay-phosphatase Complex with IR Spectroscopy and Electron Microscopy

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Title Characterization of Nanoclay-phosphatase Complex with IR Spectroscopy and Electron Microscopy
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Creator Ranjan Paul .
S.C. Datta
K.M. Manjaiah
Ranjan Bhattacharyya
 
Subject Not Available
 
Description Not Available
Commercial phosphatase enzyme (acid and alkaline) was immobilized with different nanoclays
collected from three genetically different soil orders (i.e. Inceptisol, Vertisol and Alfisol) and complexes were
characterized by IR spectroscopy and electron microscopy in the laboratory. Scanning electron microscopy
(SEM) images showed modification of surface morphologies of nanoclay due to phosphatase adsorption
(appearance of boulder like structures and rough surfaces after immobilization). IR scanning confirmed
presence of characteristic band of clay mineral due to H-O-H, Si-O stretching and Al-O-H bending in all
nanoclays. Nanoclay-phosphatase complexes were also characterized by means of IR and it was found that
carbonyl (C=O) amide III (–CO-N–) and amide I (–CO-NH2) group present in various amino acid side
chains were involved in adsorption process whereas carboxylate, primary amine and amide II (–CO-NH–)
groups present in glutamine became more free on adsorption.
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Date 2020-05-27T01:05:28Z
2020-05-27T01:05:28Z
2015-01-01
 
Type Research Paper
 
Identifier 1
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/36345
 
Language English
 
Relation Not Available;
 
Publisher Not Available