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Seasonal influences on in vitro bud break in Dendrocalamus hamiltonii Arn. ex Munro nodal explants and effect of culture microenvironment on large scale shoot multiplication and plantlet regeneration

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Title Seasonal influences on in vitro bud break in Dendrocalamus hamiltonii Arn. ex Munro nodal explants and effect of culture microenvironment on large scale shoot multiplication and plantlet regeneration
Not Available
 
Creator S. R. Singh
S. Dalal
R. Singh
A. K. Dhawan
Rajwant K. Kalia
 
Subject Ascorbic acid
axillary bud proliferation
choline chloride
commercial propagation
Dendrocalamus hamiltonii
micropropagation
thidiazuron
 
Description Not Available
A highly efficient and cost effective protocol for rapid in vitro propagation of Dendrocalamus hamiltonii
Arn. ex Munro through multiple shoot formation from nodal explants, followed by mass scale production
and field evaluation, has been developed after examining the effect of season, media type, carbon source,
growth regulators and transplanting media on micropropagation. Early summer (April-June) was the
best period for explant collection. Among the different media (B5
, MS, NN and SH) tested, MS was
found to be the best for micropropagation. A multiplication rate of about 5.6-folds with healthy cultures
was achieved by the 3rd subculture, when shoots were transferred every 3 weeks to fresh MS medium
supplemented with 1.5 μM TDZ and 56.0 μM ascorbic acid. TDZ was found superior to BAP and kinetin
for both axillary buds sprouting, as well as, shoot multiplication. Replacement of sucrose with table
sugar during shoot multiplication did not affect the multiplication frequency. Optimal rooting of 89%
was achieved on half MS medium supplemented with 25.0 μM IBA and 36.0 μM choline chloride.
Regenerated plantlets were acclimatized and hardened in green house using dune sand and vermicompost (3:1) with 79.76% success, and successfully transferred to the field with ~85% survival rate.
More than 3000 tissue culture raised plantlets have been successfully transferred to approximately
7.5 hectares of land. A cost effective method of clonal propagation of D. hamiltonii with a better field
survival rate has been developed.
Not Available
 
Date 2020-08-17T05:35:53Z
2020-08-17T05:35:53Z
2012-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/39628
 
Language English
 
Relation Not Available;
 
Publisher Not Available