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In vitro regeneration of Garcinia indica using leaf explants

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Title In vitro regeneration of Garcinia indica using leaf explants
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Creator S. K. Malik
Rajwant K. Kalia
R. Chaudhury
 
Subject In vitro regeneration
Garcinia indica
leaf lexplants
 
Description Not Available
Regeneration of plantlets via adventitious bud differentiation on leaf explants of Garcinia indica (Thouars) Choisy, a medicinally important apomictic tropical tree species was done. Leaves were cultured on Murashige and Skoog's medium supplemented with cytokinins (6-Benzylaminopurine, BAP, kinetin and Thidiazuron, TDZ) alone and in combination with auxin (a-Napthaleneacetic acid, NAA) for direct shoot proliferation. Maximum number of shoots were formed in 63.89% explants on MS medium supplemented with 1.0 ,..,M TDZ after 2-4 weeks of culture. Elongation of the induced shoots was achieved on MS basal medium containing 0.2% activated charcoal. Maximum rooting (83.33%) was observed in shoots cultured on half-strength MS medium supplemented with 10 ,..,M mA. The plantlets were successfully transferred to soil after hardening.
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Date 2020-08-17T06:41:21Z
2020-08-17T06:41:21Z
2010-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/39653
 
Language English
 
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Publisher Not Available