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Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens

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Title Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens
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Creator Kumar S
Singh G
Jat RS
Saxena SN
Sharma R
 
Subject Agrobacterium tumefaciens
cotyledonary nodes
mothbean
vacuum infiltration
Vigna aconitifolia
 
Description Not Available
The present investigation generates a rapid and convenient stable expression protocol based on vacuum infiltration of Agrobacterium tumefaciens cells into moth bean cotyledonary nodes. Vacuum infiltrated tissues showed transient GUS expression than did non infiltrated tissues. The leaflets arising from the transformed shoots on kanamycin
supplemented media were found GUS positive during histochemical assay. We believe this to be the first report of this technique for putative gene expression in mothbean cotyledonary nodes. Mothbean cotyledonary nodes are one of the most regenerative tissues in legumes.
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Date 2020-12-29T10:06:30Z
2020-12-29T10:06:30Z
2010-12-01
 
Type Research Paper
 
Identifier Kumar S, Singh G, Jat RS, Saxena SN and Sharma R. 2010. Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens. Green Farming Vol. 1(6) : 551-554.
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http://krishi.icar.gov.in/jspui/handle/123456789/44214
 
Language English
 
Relation Not Available;
 
Publisher Kumar S, Singh G, Jat RS, Saxena SN and Sharma R. 2010