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Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40

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Title Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40
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Creator Rampuria SAKSHI
Uma Joshi
Paramita Palit
Amit A. Deokar
Raju R. Meghwal
T. Mohapatra
R. Srinivasan
K.V. Bhatt
Sharma Ramavtar
 
Subject Moth bean
heat stress
SSH cDNA Library
sequence alignment
 
Description Not Available
Moth bean (Vigna aconitifolia (Jacq.) Marechal) is an important grain legume crop grown in rain fed areas of hot desert regions of Thar, India, under scorching sun rays with very little supplementation of water. An SSH cDNA library was generated from leaf tissues of V. aconitifolia var. RMO-40 exposed to an elevated temperature of 42 °C for 5 min to identify early-induced genes. A total of 488 unigenes (114 contigs and 374 singletons) were derived by cluster assembly and sequence alignment of 738 ESTs; out of 206 ESTs (28%) of unknown proteins, 160 ESTs (14%) were found to be novel to moth bean. Only 578 ESTs (78%) showed significant BLASTX similarity (
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Date 2020-12-26T11:00:49Z
2020-12-26T11:00:49Z
2012-11-02
 
Type Research Paper
 
Identifier Rampuria SAKSHI, Uma Joshi, Paramita Palit, Amit A. Deokar, Raju R. Meghwal, T. Mohapatra, R. Srinivasan, K.V. Bhatt, and Ramavtar Sharma. 2012. Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40. Genome 55:783-796
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http://krishi.icar.gov.in/jspui/handle/123456789/43786
 
Language English
 
Relation Not Available;
 
Publisher Not Available