Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40
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Title |
Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40
Not Available |
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Creator |
Rampuria SAKSHI
Uma Joshi Paramita Palit Amit A. Deokar Raju R. Meghwal T. Mohapatra R. Srinivasan K.V. Bhatt Sharma Ramavtar |
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Subject |
Moth bean
heat stress SSH cDNA Library sequence alignment |
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Description |
Not Available
Moth bean (Vigna aconitifolia (Jacq.) Marechal) is an important grain legume crop grown in rain fed areas of hot desert regions of Thar, India, under scorching sun rays with very little supplementation of water. An SSH cDNA library was generated from leaf tissues of V. aconitifolia var. RMO-40 exposed to an elevated temperature of 42 °C for 5 min to identify early-induced genes. A total of 488 unigenes (114 contigs and 374 singletons) were derived by cluster assembly and sequence alignment of 738 ESTs; out of 206 ESTs (28%) of unknown proteins, 160 ESTs (14%) were found to be novel to moth bean. Only 578 ESTs (78%) showed significant BLASTX similarity ( Not Available |
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Date |
2020-12-26T11:00:49Z
2020-12-26T11:00:49Z 2012-11-02 |
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Type |
Research Paper
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Identifier |
Rampuria SAKSHI, Uma Joshi, Paramita Palit, Amit A. Deokar, Raju R. Meghwal, T. Mohapatra, R. Srinivasan, K.V. Bhatt, and Ramavtar Sharma. 2012. Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40. Genome 55:783-796
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/43786 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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