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Polymorphism in coa and spa virulence genes in staphylococcus aureus of camel skin origin

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Title Polymorphism in coa and spa virulence genes in staphylococcus aureus of camel skin origin
Not Available
 
Creator Rathore P.
A.K. Kataria
V. Khichar
R. Sharma
 
Subject Camel
polymorphism
S. aureus
virulence genes
 
Description Not Available
The present study was undertaken with a view to identify S. aureus organisms isolated from camel skin wounds
by ribotyping and to study polymorphism in their two virulence genes viz. coa and spa, responsible for production
of coagulase and protein A, respectively. All the 15 isolates in the study were ribotyped targeting 23S rRNA gene
using species specific primers wherein a species specific amplicon of 1250 bp was produced. The polymerase chain
reaction targeting coa gene produced only single amplicon in each isolate but of variable sizes. The frequency of
amplicon of 600 bp was highest followed by amplicons of 710, 760 and 850 bp. The PCR amplification of X-region
of spa gene produced amplicons of seven different sizes viz. 160, 180, 200, 260, 280, 300 and 500 bp with calculated
number of 5, 6, 7, 9, 10, 11 and 19 repeats, respectively. Out of 15 isolates 14 produced only single amplicon whereas one isolate produced two amplicons of 200 and 500 bp size. In the present study most of the isolates possessed seven repeats of 24 bp in variable X-region of spa gene. The present investigation revealed presence of both pathogenic and nonpathogenic strains of S. aureus with polymorphism in both the virulence genes.
Not Available
 
Date 2020-12-23T10:58:00Z
2020-12-23T10:58:00Z
1001-01-01
 
Type Research Paper
 
Identifier Rathore P., A.K. Kataria, V. Khichar and R. Sharma. 2013. Polymorphism in coa and spa virulence genes in staphylococcus aureus of camel skin origin. Journal of Camel Practice and Research. Vol 19 No 2, p 129-134 (J078/6.6)
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http://krishi.icar.gov.in/jspui/handle/123456789/43593
 
Language English
 
Relation Not Available;
 
Publisher Not Available