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Development and validation of EST-derived SSR markers and diversity analysis in cluster bean (Cyamopsis tetragonoloba)

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Title Development and validation of EST-derived SSR markers and diversity analysis in cluster bean (Cyamopsis tetragonoloba)
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Creator Kumar S
Parekh MJ
Patel CB
Zala HN
Sharma R
Kulkarni KS
Fougat RS
Bhatt RK
Sakure AA
 
Subject Cluster bean
Diversity
EST-SSR
Guar gum
 
Description Not Available
Cluster bean/guar (Cyamopsis tetragonoloba), has an important place in industry because of its seeds, which contain galactomannan (guar gum) rich endosperm. Guar gum, an important ingredient of many products, is purely an export oriented commodity. Development of molecular markers for this crop is essential to accelerate breeding for guar gum content in seeds. A total of 100 novel primers pairs were developed from 16,476 expressed sequence tags (ESTs) sequence of cluster bean. A total of 50 primers pairs with function annotation of gum synthesis were selected and validated on a panel of 32 genotypes. Among the 50 primers 39 primers were amplified with a total of 45 loci. The polymorphic information content (PIC) ranged from 0.00 to 0.42 with an average of 0.13. With low polymorphic simple sequence repeats (SSRs) and narrow genetic base, most of the genotypes scattered into three clusters regardless of their geographical origin. Present study showed the existence of very low genetic diversity in cluster bean. The results indicated that there is need to explore SSR markers from whole genome or alternative marker systems like SNP (single nucleotide polymorphism) markers, for effective implication of markers in cluster bean breeding.
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Date 2020-12-30T10:56:39Z
2020-12-30T10:56:39Z
2015-10-25
 
Type Article
 
Identifier 79. Kumar S, Parekh MJ, Patel CB, Zala HN, Sharma R, Kulkarni KS, Fougat RS, Bhatt RK and Sakure AA. 2015. Development and validation of EST-derived SSR markers and diversity analysis in cluster bean (Cyamopsis tetragonoloba). J Plant Biochem Biotechnol. 25(3): 263-269
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http://krishi.icar.gov.in/jspui/handle/123456789/44296
 
Language English
 
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Publisher Not Available