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Molecular typing of Indian Mycoplasma gallisepticum isolates

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Title Molecular typing of Indian Mycoplasma gallisepticum isolates
Not Available
 
Creator Susitha Rajkumar
Maddula Ramkoti Reddy
Ramesh Somvanshi
 
Subject M. gallisepticum, mgc2 gene, Sequencing, Phylogenetic analysis, Poultry, RFLP
 
Description Not Available
M. gallisepticum infection is a serious economic threat to the poultry industry in India, and the genetic diversity of Indian
isolates is unknown. Gene-targeted sequence analysis of M. gallisepticum was conducted for the first time to map the
diversity among different Indian isolates from different regions and from different clinical conditions. Sequence analysis of
the mgc2 gene grouped the 13 field isolates into four groups. The most prevalent sequence type, the group1 shared 100%
identity with the foreign strains THA 3CK08 (Thailand) and UHP1CK99 (Israel). Groups two and three were novel, and were
genetically distinct from the strains outside the country. Group four shared 100% similarity with the vaccine strain MG6/85.
The nucleotide similarity between the 4 groups ranged from 94.3-99.2%. A PCR-RFLP method using HaeII was tested and
validated for its ability to differentiate the Indian field isolates from vaccine strains.
ICAR funded Institute Project at ICAR-DPR
 
Date 2021-03-09T05:24:26Z
2021-03-09T05:24:26Z
2019-12-01
 
Type Journal
 
Identifier Rajkumar, S., Reddy, M. R., & Somvanshi, R. (2019). Molecular typing of Indian Mycoplasma gallisepticum isolates. Indian Journal of Animal Research, 53(12):1645-1650.
0367-6722
http://krishi.icar.gov.in/jspui/handle/123456789/45989
 
Language English
 
Relation Not Available;
 
Publisher AGRICULTURAL RESEARCH COMMUNICATION CENTRE