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Molecular Typing of Indian Mycoplasma synoviae Isolates

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Title Molecular Typing of Indian Mycoplasma synoviae Isolates
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Creator Susitha Rajkumar
Maddula Ramkoti Reddy
Ramesh Somvanshi
 
Subject M. synoviae, Nucleotide polymorphism, Phylogenetic analysis, Poultry, Proline rich repeat, vlhA gene
 
Description Not Available
Background: Mycoplasma synoviae is an economically significant pathogen in poultry with reported increased prevalence and
virulence in recent years. The pathogen causes subclinical upper respiratory tract infection, air sacculitis and infectious synovitis.
Methods: This study aimed to characterize the field isolates of M. synoviae, from healthy and diseased birds of 13 poultry flocks of
five states of India by DNA sequence analysis of the conserved 52 region of variable lipoprotein and haemagglutinin (vlhA) gene.
Result: Phylogenetic analysis revealed 7 sequence types. Single sequence type was found in three major states for commercial
poultry production, which could be due to interstate transport of birds or chicks.Typing based on nucleotide insertion/deletion in the
proline-rich repeat (PRR) region and the nucleotide polymorphisms of the RIII region of the 52 region of the vlhA identified field
isolates as Type C (n=8), E (n=6) and L (n=5). Subtypes identified were C1 (n=3) and C3 (n=1) and others were novel. Most of the
isolates were from birds having respiratory lesions or symptoms. Synovitis cases were very rare and the isolate from synovitis was
found to be of subtype C3. Further, the presence of multiple types (C1, E and L) was found in one farm even though most of the farms
were affected by only single type. Molecular typing of Indian M. synoviae isolateswas conducted for the first time to map the diversity
among different Indian isolates from different regions and from different clinical conditions.
ICAR funded Institute Project at ICAR-DPR
 
Date 2021-03-09T05:24:45Z
2021-03-09T05:24:45Z
2020-11-20
 
Type Journal
 
Identifier Rajkumar, S., Reddy, M. R., & Somvanshi, R. (2019). Molecular typing of Indian Mycoplasma synoviae isolates. Indian Journal of Animal Research DOI: 10.18805/IJAR.B-4153
0367-6722
http://krishi.icar.gov.in/jspui/handle/123456789/45990
 
Language English
 
Relation Not Available;
 
Publisher AGRICULTURAL RESEARCH COMMUNICATION CENTRE